Cat. No.
10230-01

Size
0.5 mg

Price (USD)
$200.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	MP6-XT22
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-UNLB
Immunogen	E. coli-expressed mouse TNF-α
Specificity	Mouse/Chicken TNF-α
Alternalte Name(s)	Tumor necrosis factor-α, macrophage cytotoxic factor , MCF, differentiation inducing factor, DIF, cachectin, necrosin, TNFSF-2
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Capture – Quality tested ^2,3 ELISA-Detection – Quality tested ^1,4 Flow Cytometry – Quality tested ^6-12 ELISpot-Capture – Reported in literature ⁵ Immunohistochemsitry-Frozen Sections – Reported in literature ^13-19 Immunohistochemsitry-Paraffin Sections – Reported in literature ¹⁶ Immunocytochemistry – Reported in literature ^20,21 Western Blot – Reported in literature ²¹ Neutralization – Reported in literature ^1,22-24 Multiplex-Detection – Reported in literature ²⁵
Recommended Dilutions	Please refer to product specific Technical Bulletin

References

1.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2.	Heinzel FP, Rerko RM, Ahmed F, Hujer AM. IFN-γ-independent production of IL-12 during murine endotoxemia. J Immunol. 1996;157:4521-8. (ELISA-Capture)
3.	Bordmann G, Rudin W, Favre N. Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections. Immunology. 1998;94:35-40. (ELISA-Capture)
4.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
5.	Favre N, Bordmann G, Rudin W. Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J Immunol Methods. 1997;204:57-66. (ELISPOT-Capture)
6.	Tripp RA, Moore D, Jones L, Sullender W, Winter J, Anderson LJ. Respiratory syncytial virus G and/or SH protein alters Th1 cytokines, natural killer cells, and neutrophils responding to pulmonary infection in BALB/c mice. J Virol. 1999;73:7099-107. (FC)
7.	Asseman C, Mauze S, Leach MW, Coffman RL, Powrie F. An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. J Exp Med. 1999;190:995-1003. (FC)
8.	Satoh M, Yasunami Y, Matsuoka N, Nakano M, Itoh T, Nitta T, et al. Successful islet transplantation to two recipients from a single donor by targeting proinflammatory cytokines in mice. Transplantation. 2007;83:1085-92. (FC)
9.	Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
10.	Voß EV, Škuljec J, Gudi V, Skripuletz R, Pul R, Trebst C, et al. Characterisation of microglia during de- and remyelination: can they create a repair promoting environment?. Neurobiol Dis. 2012;45:519-28. (FC)
11.	Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
12.	Nakamura T, Nakao T, Yoshimura N, Ashihara E. Rapamycin prolongs cardiac allograft survival in a mouse model by inducing myeloid-derived suppressor cells. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC)
13.	Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
14.	Sunnemark D, Ulfgren A, Örn A, Harris RA. Cytokine production in hearts of Trypanosoma cruzi-infected CBA mice: Do cytokine patterns in chronic stage reflect the establishment of myocardial pathology?. Scand J Immunol. 1996;44:421-9. (IHC-FS)
15.	Marinova-Mutafchieva L, Williams RO, Mason LJ, Mauri C, Feldmann M, Maini RN. Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). Clin Exp Immunol. 1997;107:507-12. (IHC-FS)
16.	Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS)
17.	Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
18.	Jacobs M, Brown N, Allie N, Gulert R, Ryffel B. Increased resistance to mycobacterial infection in the absence of interleukin-10. Immunology. 2000;100:494-501. (IHC-FS)
19.	Williams RO, Marinova-Mutafchieva L, Feldmann M, Maini RN. Evaluation of TNF-α and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-α/anti-CD4 therapy. J Immunol. 2000;165:7240-5. (IHC-FS)
20.	Sanders EJ, Prasad S, Hu N, Wride MA. Cell death in the gastrulating chick embryo: potential roles for tumor necrosis factor-alpha (TNF-α). Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity)
21.	Wride MA, Sanders EJ. Expression of tumor necrosis factor-α (TNFα)-cross-reactive proteins during early chick embryo development. Dev Dyn. 1993;198:225-39. (ICC, WB, Chicken Reactivity)
22.	Scanga CA, Mohan VP, Joseph H, Yu K, Chan J, Flynn JL. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun. 1999;67:4531-8. (Neut)
23.	Via CS, Shustov A, Rus V, Lang T, Nguyen P, Finkelman FD. In vivo neutralization of TNF-α promotes humoral autoimmunity by preventing the induction of CTL. J Immunol. 2001;167:6821-6. (Neut)
24.	Zhou R, Wei H, Sun R, Tian Z. Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007;178:4548-56. (Neut)
25.	Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)

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Rat Anti-Mouse TNF-α-UNLB