Rat Anti-Mouse TNF-α-UNLB

Cat. No.
10230-01  
Size
0.5 mg   		Price (USD)
$200.00 
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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone MP6-XT22
Isotype Rat IgG1κ
Isotype Control Rat IgG1-UNLB
Immunogen E. coli-expressed mouse TNF-α
Specificity Mouse/Chicken TNF-α
Alternalte Name(s) Tumor necrosis factor-α, macrophage cytotoxic factor , MCF, differentiation inducing factor, DIF, cachectin, necrosin, TNFSF-2
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 2,3
ELISA-Detection – Quality tested 1,4
Flow Cytometry – Quality tested 6-12
ELISpot-Capture – Reported in literature 5
Immunohistochemsitry-Frozen Sections – Reported in literature 13-19
Immunohistochemsitry-Paraffin Sections – Reported in literature 16
Immunocytochemistry – Reported in literature 20,21
Western Blot – Reported in literature 21
Neutralization – Reported in literature 1,22-24
Multiplex-Detection – Reported in literature 25
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794273
Gene ID
21926 (Mouse)
Gene Symbol
Tnf (Mouse)
Gene Aliases DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Uniprot ID
P06804 (Mouse)
Uniprot Name
TNFA_MOUSE 
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References
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11. Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
12. Nakamura T, Nakao T, Yoshimura N, Ashihara E. Rapamycin prolongs cardiac allograft survival in a mouse model by inducing myeloid-derived suppressor cells. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC)
13. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
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15. Marinova-Mutafchieva L, Williams RO, Mason LJ, Mauri C, Feldmann M, Maini RN. Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). Clin Exp Immunol. 1997;107:507-12. (IHC-FS)
16. Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS)
17. Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
18. Jacobs M, Brown N, Allie N, Gulert R, Ryffel B. Increased resistance to mycobacterial infection in the absence of interleukin-10. Immunology. 2000;100:494-501. (IHC-FS)
19. Williams RO, Marinova-Mutafchieva L, Feldmann M, Maini RN. Evaluation of TNF-α and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-α/anti-CD4 therapy. J Immunol. 2000;165:7240-5. (IHC-FS)
20. Sanders EJ, Prasad S, Hu N, Wride MA. Cell death in the gastrulating chick embryo: potential roles for tumor necrosis factor-alpha (TNF-α). Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity)
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22. Scanga CA, Mohan VP, Joseph H, Yu K, Chan J, Flynn JL. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun. 1999;67:4531-8. (Neut)
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24. Zhou R, Wei H, Sun R, Tian Z. Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007;178:4548-56. (Neut)
25. Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)