Rat Anti-Mouse TNF-α-BIOT (MP6-XT22)
Cat. No.:
10230-08
Biotin Anti-Mouse TNF-α antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, ELISA, and multiplex assays.
$253.00
Clone | MP6-XT22 |
---|---|
Isotype | Rat IgG1κ |
Isotype Control | Rat IgG1-BIOT (KLH/G1-2-2) |
Specificity | Mouse/Chicken TNF-α |
Alternative Names | Tumor necrosis factor-α, macrophage cytotoxic factor , MCF, differentiation inducing factor, DIF, cachectin, necrosin, TNFSF-2 |
Immunogen | E. coli-expressed mouse TNF-α |
Conjugate | BIOT (Biotin) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C |
Applications |
ELISA-Capture – Quality tested 2,3 ELISA-Detection – Quality tested 1,4 Flow Cytometry – Quality tested 6-12 ELISpot-Capture – Reported in literature 5 Immunohistochemsitry-Frozen Sections – Reported in literature 13-19 Immunohistochemsitry-Paraffin Sections – Reported in literature 16 Immunocytochemistry – Reported in literature 20,21 Western Blot – Reported in literature 21 Neutralization – Reported in literature 1,22-24 Multiplex-Detection – Reported in literature 25 |
RRID Number | AB_2794275 |
Gene ID |
21926 (Mouse) |
Gene ID Symbol |
Tnf (Mouse) |
Gene ID Aliases | DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha |
UniProt ID |
P06804 (Mouse |
UniProt Name |
TNFA_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
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- 13. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
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- 15. Marinova-Mutafchieva L, Williams RO, Mason LJ, Mauri C, Feldmann M, Maini RN. Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). Clin Exp Immunol. 1997;107:507-12. (IHC-FS)
- 16. Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS)
- 17. Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
- 18. Jacobs M, Brown N, Allie N, Gulert R, Ryffel B. Increased resistance to mycobacterial infection in the absence of interleukin-10. Immunology. 2000;100:494-501. (IHC-FS)
- 19. Williams RO, Marinova-Mutafchieva L, Feldmann M, Maini RN. Evaluation of TNF-α and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-α/anti-CD4 therapy. J Immunol. 2000;165:7240-5. (IHC-FS)
- 20. Sanders EJ, Prasad S, Hu N, Wride MA. Cell death in the gastrulating chick embryo: potential roles for tumor necrosis factor-alpha (TNF-α). Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity)
- 21. Wride MA, Sanders EJ. Expression of tumor necrosis factor-α (TNFα)-cross-reactive proteins during early chick embryo development. Dev Dyn. 1993;198:225-39. (ICC, WB, Chicken Reactivity)
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- 24. Zhou R, Wei H, Sun R, Tian Z. Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007;178:4548-56. (Neut)
- 25. Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)
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