Cat. No.
10236-08

Size
0.5 mg

Price (USD)
$230.00

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Technical Bulletin Certificate of
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Clone	MP1-31G6
Isotype	Rat IgG₁κ
Isotype Control	Rat IgG₁-BIOT
Immunogen	COS-expressed mouse GM-CSF
Specificity	Mouse GM-CSF
Alternalte Name(s)	Granulocyte/macrophage colony-stimulating factor, GM CSF, CSF-α, pluripoietin-α, eosinophil colony stimulating factor, Eo-CSF, burst promoting activity, BPA
Format/Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA-Detection – Quality tested ^1-4 Immunohistochemsitry-Frozen Sections – Reported in literature ⁵ Immunocytochemistry – Reported in literature ^1,5 Immunoprecipitation – Reported in literature ² Neutralization – Reported in literature ^1,2 Multiplex-Detection – Reported in literature ³ Note – May be paired with the purified clone MP1-22E9 (SB Cat. No. 10235-01) in a sandwich ELISA
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794282

Standard curve generated with Rat Anti-Mouse GM-CSF-UNLB (SB Cat. No. 10235-01; Clone MP1-22E9) and Rat Anti-Mouse GM-CSF-BIOT (SB Cat. No. 10236-08; Clone MP1-31G6) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

Standard curve generated with Rat Anti-Mouse GM-CSF-UNLB (SB Cat. No. 10235-01; Clone MP1-22E9) and Rat Anti-Mouse GM-CSF-BIOT (SB Cat. No. 10236-08; Clone MP1-31G6) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

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References

1.	Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (Immunogen, ELISA-Detection, ICC, Neut)
2.	Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, IP, Neut)
3.	Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Detection, Multiplex-Detection)
4.	Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
5.	Yokoyama H, Naito T, Wada T, Kelley VR. Application of genetically engineered tubular epithelial cells in kidney disease. Exp Nephrol. 1999;7:267-72. (IHC-FS, ICC)

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