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  • Goat Anti-Mouse IgG Fc-UNLB

Goat Anti-Mouse IgG Fc-UNLB

Cat. No.:
1033-01
Purified Goat Anti-Mouse IgG Fc antibody for use in ELISA and western blot assays.
$76.00
Size Price (USD) Quantity
1.0 mg $76.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with the Fc region of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Mouse IgG Fab, IgM, and IgA; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA

Other referenced applications for relevant formats include -
Western Blot 2
RRID Number AB_2794330

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Cabral TM, Baig A, Berhane Y, Schmidt L, Hole K, Leith M, et al. Development of neutralizing monoclonal antibodies against the pandemic H1N1 virus (2009) using plasmid DNA immunogen. J Virol Methods. 2014;195:54-62. (ELISA)
  2. 2. Lin L, Pan S, Zhao J, Liu C, Wang P, Fu L, et al. HSPD1 interacts with IRF3 to facilitate interferon-beta induction. PLoS One. 2014;9(12):e114874. (WB)

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  1. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  2. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  3. ELISA plate was coated with purified mouse IgG, IgM, IgA, IgG Fab, and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG Fab-UNLB (SB Cat. No. 1015-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Mouse IgG Fab-UNLB
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  4. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115h)
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  5. Supernatant fractions from Bax-/- mouse embryonic fibroblasts were incubated with caspase-8-cleaved human Bid (tBid) and anti-Bak (Clone 7D10 and Clone 8F8), resolved by electrophoresis, transferred to membrane, and probed with anti-cytochrome c followed by Goat Anti-Mouse Ig, Human ads-HRP (SB Cat. No. 1010-05) and chemiluminescent detection.<br/>Image from Iyer S, Anwari K, Alsop AE, Yuen WS, Huang DC, Carroll J, et al. Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nat Commun. 2016;7:11734. Figure 1(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Goat Anti-Mouse Ig, Human ads-HRP
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  6. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  7. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 6425-30).
    Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488
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  8. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  9. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  10. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF555 (SB Cat. No. 6420-32) and DAPI.
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