Goat Anti-Mouse IgG Fc-BIOT

Cat. No.
1033-08  
Size
1.0 mg  
Price (USD)
$80.00 
Specificity Reacts with the Fc region of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Mouse IgG Fab, IgM, and IgA; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Western Blot 2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG Fc-BIOT (SB Cat. No. 1033-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified mouse IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG Fc-BIOT (SB Cat. No. 1033-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
References
1. Cabral TM, Baig A, Berhane Y, Schmidt L, Hole K, Leith M, et al. Development of neutralizing monoclonal antibodies against the pandemic H1N1 virus (2009) using plasmid DNA immunogen. J Virol Methods. 2014;195:54-62. (ELISA)
2. Lin L, Pan S, Zhao J, Liu C, Wang P, Fu L, et al. HSPD1 interacts with IRF3 to facilitate interferon-beta induction. PLoS One. 2014;9(12):e114874. (WB)