Mouse Anti-Human EGFR-UNLB

Cat. No.
10400-01  
Size
0.1 mg  
Price (USD)
$205.00 
Clone SB41a
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen Recombinant human EGFR
Specificity Human EGFR
Alternalte Name(s) Epidermal growth factor receptor, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, ErbB-1, HER1
Description Epidermal growth factor receptor (EGFR) is a transmembrane receptor for epidermal growth factor (EGF). It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases - HER1 (EGFR, ErbB-1), HER (Neu, ErbB-2), HER3 (ErbB-3), and HER4 (ErbB-4). EGFR exists on the cell surface as inactive monomers and is activated by binding of its specific ligands, including EGF, transforming growth factor alpha (TGF-α), or others. Upon activation, EGFR can pair with another EGFR to form an active homodimer or pair with another member of the ErbB receptor family to create a heterodimer.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Total cell lysates from HEp-2 (Lane 1) and MIA PaCa-2 (Lane 2) cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
  • Human epithelial carcinoma cell line HEp-2 was stained with with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-CY3 (SB Cat. No. 7100-12), Rat Anti-β-Actin-FITC, and DAPI.
  • Paraffin embedded human gastric cancer tissue was stained with Mouse IgG-UNLB isotype control (SB Cat. No. 0107-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by an HRP conjugated anti-mouse IgG secondary antibody, DAB, and hematoxylin.
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01) followed by Goat Anti-Mouse IgG, Human ads-AF488 (SB Cat. No. 1030-30) and DAPI.
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04), Red AP, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Total cell lysates from HEp-2 (Lane 1) and MIA PaCa-2 (Lane 2) cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 

Human epithelial carcinoma cell line HEp-2 was stained with with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-CY3 (SB Cat. No. 7100-12), Rat Anti-β-Actin-FITC, and DAPI.
 

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG-UNLB isotype control (SB Cat. No. 0107-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by an HRP conjugated anti-mouse IgG secondary antibody, DAB, and hematoxylin.
 

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01) followed by Goat Anti-Mouse IgG, Human ads-AF488 (SB Cat. No. 1030-30) and DAPI.
 

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04), Red AP, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
 
References
1. SouthernBiotech published data (IHC-PS, ICC)