Unlabeled Anti-Human EGFR, SB41a

Cat. No.
10400-01

Size
0.1 mg

Price (USD)
$205.00

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Clone	SB41a
Isotype	Mouse (BALB/c) IgG_2aκ
Isotype Control	Mouse IgG_2a-UNLB
Immunogen	Recombinant human EGFR
Specificity	Human EGFR
Alternalte Name(s)	Epidermal growth factor receptor, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, ErbB-1, HER1
Description	Epidermal growth factor receptor (EGFR) is a transmembrane receptor for epidermal growth factor (EGF). It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases - HER1 (EGFR, ErbB-1), HER (Neu, ErbB-2), HER3 (ErbB-3), and HER4 (ErbB-4). EGFR exists on the cell surface as inactive monomers and is activated by binding of its specific ligands, including EGF, transforming growth factor alpha (TGF-α), or others. Upon activation, EGFR can pair with another EGFR to form an active homodimer or pair with another member of the ErbB receptor family to create a heterodimer.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Western Blot – Quality tested Immunohistochemistry-Paraffin Sections ¹ Immunocytochemistry ¹
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794369

Gene ID	1956 (Mouse)
Gene Symbol	EGFR (Mouse)
Gene Aliases	ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
Uniprot ID	P00533 (Human)
Uniprot Name	EGFR_HUMAN

Total cell lysates from HEp-2 (Lane 1) and MIA PaCa-2 (Lane 2) cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
Human epithelial carcinoma cell line HEp-2 was stained with with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-CY3 (SB Cat. No. 7100-12), Rat Anti-β-Actin-FITC, and DAPI.
Paraffin embedded human gastric cancer tissue was stained with Mouse IgG-UNLB isotype control (SB Cat. No. 0107-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by an HRP conjugated anti-mouse IgG secondary antibody, DAB, and hematoxylin.
Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01) followed by Goat Anti-Mouse IgG, Human ads-AF488 (SB Cat. No. 1030-30) and DAPI.
Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04), Red AP, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Total cell lysates from HEp-2 (Lane 1) and MIA PaCa-2 (Lane 2) cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Human epithelial carcinoma cell line HEp-2 was stained with with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-CY3 (SB Cat. No. 7100-12), Rat Anti-β-Actin-FITC, and DAPI.

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG-UNLB isotype control (SB Cat. No. 0107-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by an HRP conjugated anti-mouse IgG secondary antibody, DAB, and hematoxylin.

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01) followed by Goat Anti-Mouse IgG, Human ads-AF488 (SB Cat. No. 1030-30) and DAPI.

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04), Red AP, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).