| Clone |
SB41a
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| Isotype |
Mouse (BALB/c) IgG2aκ
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| Isotype Control |
Mouse IgG2a-UNLB
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| Immunogen |
Recombinant human EGFR
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| Specificity |
Human EGFR
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| Alternalte Name(s) |
Epidermal growth factor receptor, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, ErbB-1, HER1
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| Description |
Epidermal growth factor receptor (EGFR) is a transmembrane receptor for epidermal growth factor (EGF). It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases - HER1 (EGFR, ErbB-1), HER (Neu, ErbB-2), HER3 (ErbB-3), and HER4 (ErbB-4). EGFR exists on the cell surface as inactive monomers and is activated by binding of its specific ligands, including EGF, transforming growth factor alpha (TGF-α), or others. Upon activation, EGFR can pair with another EGFR to form an active homodimer or pair with another member of the ErbB receptor family to create a heterodimer.
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| Format/Conjugate |
UNLB (Unconjugated)
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| Buffer Formulation |
Borate buffered saline, pH 8.2
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| Concentration |
0.1 mg/mL
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| Volume |
1.0 mL
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| Storage & Handling |
2-8°C
Please refer to product specific SDS
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| Applications |
Applications for relevant formats of this clone include - Western Blot – Quality tested
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
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| Recommended Dilutions |
Please refer to product specific Technical Bulletin
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Total cell lysates from HEp-2 (Lane 1) and MIA PaCa-2 (Lane 2) cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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Human epithelial carcinoma cell line HEp-2 was stained with with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-CY3 (SB Cat. No. 7100-12), Rat Anti-β-Actin-FITC, and DAPI.
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Paraffin embedded human gastric cancer tissue was stained with Mouse IgG-UNLB isotype control (SB Cat. No. 0107-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by an HRP conjugated anti-mouse IgG secondary antibody, DAB, and hematoxylin.
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Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01) followed by Goat Anti-Mouse IgG, Human ads-AF488 (SB Cat. No. 1030-30) and DAPI.
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Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04), Red AP, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Total cell lysates from HEp-2 (Lane 1) and MIA PaCa-2 (Lane 2) cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
Human epithelial carcinoma cell line HEp-2 was stained with with Mouse IgG2a-UNLB isotype control (SB Cat. No. 0103-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-CY3 (SB Cat. No. 7100-12), Rat Anti-β-Actin-FITC, and DAPI.
Paraffin embedded human gastric cancer tissue was stained with Mouse IgG-UNLB isotype control (SB Cat. No. 0107-01; left) and Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by an HRP conjugated anti-mouse IgG secondary antibody, DAB, and hematoxylin.
Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01) followed by Goat Anti-Mouse IgG, Human ads-AF488 (SB Cat. No. 1030-30) and DAPI.
Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human EGFR-UNLB (SB Cat. No. 10400-01; right) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04), Red AP, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
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