Goat F(ab')2 Anti-Mouse Kappa-BIOT

Cat. No.
1052-08  
Size
0.5 mg  
Price (USD)
$195.00 
Specificity Reacts with mouse κ light chains
Source Pepsin digest of Goat Anti-Mouse Kappa (SB Cat. No. 1050)
Cross Adsorption Mouse λ light chains; may react with κ light chains from other species
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
Flow Cytometry
Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 8
ELISpot 1
Stimulation 2-7
Recommended Dilutions Please refer to product specific Technical Bulletin
  • C57BL/6 mouse splenocytes were stained with Goat F(ab’)2 Anti-Mouse Kappa-BIOT (SB Cat. No. 1052-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
  • ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Mouse Kappa-BIOT (SB Cat. No. 1052-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

C57BL/6 mouse splenocytes were stained with Goat F(ab’)2 Anti-Mouse Kappa-BIOT (SB Cat. No. 1052-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 

ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Mouse Kappa-BIOT (SB Cat. No. 1052-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Velez M, Kane M, Liu S, Gauld SB, Cambier JC, Torres RM, et al. Ig allotypic inclusion does not prevent B cell development or response. J Immunol. 2007;179:1049-57. (ELISA, ELISPOT)
2. Donahue AC, Fruman DA. Distinct signaling mechanisms activate the target of rapamycin in response to different B-cell stimuli. Eur J Immunol. 2007;37:2923-36. (Stim)
3. Hobeika E, Levit-Zerdoun E, Anastasopoulou V, Pohlmeyer R, Altmeier S, Alsadeq A, et al. CD19 and BAFF-R can signal to promote B-cell survival in the absence of Syk. EMBO J. 2015;34:925-39. (Stim)
4. Ouchida R, Lu Q, Liu J, Li Y, Chu Y, Tsubata T, et al. FcμR interacts and cooperates with the B cell receptor to promote B cell survival. J Immunol. 2015;194:3096-101. (Stim)
5. Levit-Zerdoun E, Becker N, Pohlmeyer R, Wilhelm I, Maity PC, Rajewsky K, et al. Survival of Igα-deficient mature B cells requires BAFF-R function. J Immuol. 2016;196:2348-60. (Stim)
6. Yasuda S, Sun J, Zhou Y, Wang Y, Lu Q, Yamamura M, et al. Opposing roles of IgM and IgD in BCR-induced B-cell survival. Genes Cells. 2018;23:868-79. (Stim)
7. Noviski M, Mueller JL, Satterthwaite A, Garrett-Sinha LA, Brombacher F, Zikherman J. IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate. Elife. 2018;7:e35074. (Stim)
8. SouthernBiotech published data (IHC-PS)