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Mouse Anti-Influenza A, Nucleoprotein-UNLB

Cat. No.
10780-01  
Size
0.5 mg  
Price (USD)
$185.00 
Qty
Add to Cart
Clone FluA-NP 4F1
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Recombinant influenza virus type A nucleoprotein
Specificity Influenza virus type A nucleoprotein
Alternalte Name(s) NP
Description Influenza virus type A nucleoprotein, also known as NP, is composed of a 498 amino acid sequence and is type-specific in influenza viruses. The NP encapsulates the virus genome to form a ribonucleoprotein (RNP) particle for the purposes of transcription and packaging. The monoclonal antibody FluA-NP 4F1 has been shown to specifically recognize type A influenza virus NP in virus infected cell culture and patient samples. This clone can be paired as a detection antibody for ELISA with clone FluA-NP 2C9 (SB Cat. No. 10770-01).
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Immunocytochemistry – Reported in literature 1-4
Immunoblotting – Reported in literature 2,4,5
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Recombinant influenza proteins were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) and with chemiluminescent detection.
  • MDCK cells infected with the influenza A (H1N1) virus were stained with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01) followed by a secondary antibody and DAPI.

    Image from Shoji M, Woo S, Masuda A, Win NN, Ngwe H, Takahashi E, et al. Anti-influenza virus activity of extracts from the stems of Jatropha multifida Linn. collected in Myanmar. BMC Complement Altern Med. 2017;17:96. Figure 3(a)

Recombinant influenza proteins were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) and with chemiluminescent detection.
 

MDCK cells infected with the influenza A (H1N1) virus were stained with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01) followed by a secondary antibody and DAPI.

Image from Shoji M, Woo S, Masuda A, Win NN, Ngwe H, Takahashi E, et al. Anti-influenza virus activity of extracts from the stems of Jatropha multifida Linn. collected in Myanmar. BMC Complement Altern Med. 2017;17:96. Figure 3(a)
 
References
1. Wei G, Meng W, Guo H, Pan W, Liu J, Peng T, et al. Potent neutralization of influenza A virus by a single-domain antibody blocking M2 ion channel protein. PLoS One. 2011;6(12):e28309. (ICC)
2. Shoji M, Takahashi E, Hatakeyama D, Iwai Y, Morita Y, Shirayama R, et al. Anti-influenza activity of C60 fullerene derivatives. PLoS One. 2013;8(6):e66337. (ICC, WB)
3. Hatakeyama D, Shoji M, Yamayoshi S, Hirota T, Nagae M, Yanagisawa S, et al. A novel functional site in the PB2 subunit of influenza A virus essential for acetyl-CoA interaction, RNA polymerase activity, and viral replication. J Biol Chem. 2014;289:24980-94. (ICC)
4. Shoji M, Arakaki Y, Esumi T, Kohnomi S, Yamamoto C, Suzuki Y, et al. Bakuchiol is a phenolic isoprenoid with novel enantiomer-selective anti-influenza A virus activity involving Nrf2 activation. J Biol Chem. 2015 Oct 7. pii: jbc.M115.669465. [Epub ahead of print]. (ICC, WB)
5. Narkpuk J, Wanitchang A, Kramyu J, Frantz PN, Jongkaewwattana A, Teeravechyan S. An unconventional BST-2 function: down-regulation of transient protein expression. Biochem Biophys Res Commun. 2014;450:1469-74. (WB)
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