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Goat Anti-Mouse IgG2a, Human ads-TRITC

Cat. No.
1080-03  
Size
1.0 mg  
Price (USD)
$200.00 
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Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Source Pooled antisera from goats hyperimmunized with mouse IgG2a paraproteins
Cross Adsorption Mouse IgM, IgG1, IgG2b, IgG3 and IgA; pooled human sera; purified human paraproteins
Purification Affinity chromatography on mouse IgG2a covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-TRITC
Format/Conjugate TRITC (Rhodamine)
Buffer Formulation Supplied in PBS/NaN3
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections
Immunohistochemistry-Paraffin Sections
Immunocytochemistry
Immunoblotting
ELISpot
Purification
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-TRITC (SB Cat. No. 1080-03).
  • Frozen equine sacroid tissue section was stained with anti-MHC I followed by Goat Anti-Mouse IgG2a, Human ads-TRITC (SB Cat. No. 1080-03) and DAPI.

    Image from Wilson AD, Hicks C. Both tumour cells and infiltrating T-cells in equine sarcoids express FOXP3 associated with an immune-supressed cytokine microenvironment. Vet Res. 2016;47:55. Figure 5(h)

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-TRITC (SB Cat. No. 1080-03).
 

Frozen equine sacroid tissue section was stained with anti-MHC I followed by Goat Anti-Mouse IgG2a, Human ads-TRITC (SB Cat. No. 1080-03) and DAPI.

Image from Wilson AD, Hicks C. Both tumour cells and infiltrating T-cells in equine sarcoids express FOXP3 associated with an immune-supressed cytokine microenvironment. Vet Res. 2016;47:55. Figure 5(h)
 
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