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Goat Anti-Mouse IgG2a, Human ads-HRP

Cat. No.
1080-05  
Size
1.0 mL  
Price (USD)
$120.00 
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Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Source Pooled antisera from goats hyperimmunized with mouse IgG2a paraproteins
Cross Adsorption Mouse IgM, IgG1, IgG2b, IgG3 and IgA; pooled human sera; purified human paraproteins
Purification Affinity chromatography on mouse IgG2a covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections
Immunohistochemistry-Paraffin Sections
Immunocytochemistry
Immunoblotting
ELISpot
Purification
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05)..
  • Total cell lysates from mouse thymocytes and Hep G2 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-xL-UNLB (SB Cat. No. 10035-01). Proteins were visualized using Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.
  • Human kidney cancer tissue was stained with Mouse Anti-Human MMP-3-UNLB (SB Cat. No. 12020-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05).

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05)..
 

Total cell lysates from mouse thymocytes and Hep G2 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-xL-UNLB (SB Cat. No. 10035-01). Proteins were visualized using Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.
 

Human kidney cancer tissue was stained with Mouse Anti-Human MMP-3-UNLB (SB Cat. No. 12020-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05).
 
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