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Goat Anti-Mouse IgG2a, Human ads-TXRD

Cat. No.
1080-07  
Size
1.0 mg  
Price (USD)
$210.00 
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Specificity Reacts with the heavy chain of mouse IgG2a
Source Pooled antisera from goats hyperimmunized with mouse IgG2a
Cross Adsorption Mouse IgG1, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG2a covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-TXRD
Format/Conjugate TXRD (Texas Red®)
Buffer Formulation Supplied in PBS/NaN3
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-9
FLISA
Flow Cytometry 11-13
Other referenced applications for relevant formats include -
ELISpot 3,10
Immunohistochemistry-Frozen Sections 2,14,15
Immunohistochemistry-Paraffin Sections 16,17
Immunocytochemistry 4,18-20
Electron Microscopy 14
Western Blot 1,21-23
Surface Plasmon Resonance 24
Purification 25
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-TXRD (SB Cat. No. 1080-07).
  • Human myofibroblasts were stained with anti-COX2 and anti-α-SMA followed by Goat Anti-Mouse IgG2a, Human ads-TXRD (SB Cat. No. 1080-07), Goat Anti-Mouse IgG1, Human ads-FITC (SB Cat. No. 1070-02), and DAPI.

    Image from Mattyasovszky SG, Hofmann A, Brochhausen C, Ritz U, Kuhn S, Wollstädter J, et al. The effect of the pro-inflammatory cytokine tumor necrosis factor-alpha on human joint capsule myofibroblasts. Arthritis Res Ther. 2010;12:R4. Figure 7(a)

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-TXRD (SB Cat. No. 1080-07).
 

Human myofibroblasts were stained with anti-COX2 and anti-α-SMA followed by Goat Anti-Mouse IgG2a, Human ads-TXRD (SB Cat. No. 1080-07), Goat Anti-Mouse IgG1, Human ads-FITC (SB Cat. No. 1070-02), and DAPI.

Image from Mattyasovszky SG, Hofmann A, Brochhausen C, Ritz U, Kuhn S, Wollstädter J, et al. The effect of the pro-inflammatory cytokine tumor necrosis factor-alpha on human joint capsule myofibroblasts. Arthritis Res Ther. 2010;12:R4. Figure 7(a)
 
References
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3. Cox RJ, Mykkeltvedt E, Robertson J, Haaheim LR. Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication. Scand J Immunol. 2002;55:14-23. (ELISA, ELISPOT)
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11. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
12. Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, et al. Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β. Nature. 2012;484:514-8. (FC)
13. Sutton KM, Hu T, Wu Z, Siklodi B, Vervelde L, Kaiser P. The functions of the avian receptor activator of NF-κB ligand (RANKL) and its receptors, RANK and osteoprotegerin, are evolutionarily conserved. Dev Comp Immunol. 2015;51:170-84. (FC)
14. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
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17. Kreijtz JH, Wiersma LC, De Gruyter HL, Vogelzang-van Trierum SE, van Amerongen G, Stittelaar KJ, et al. A single immunization with modified vaccinia virus Ankara-based influenza virus H7 vaccine affords protection in the influenza A(H7N9) pneumonia ferret model. J Infect Dis. 2015;211:791-800. (IHC-PS)
18. Albrecht B, Woisetschläger M, Robertson MW. Export of the high affinity IgE receptor from the endoplasmic reticulum depends on a glycosylation-mediated quality control mechanism. J Immunol. 2000;165:5686-94. (ICC)
19. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
20. Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)
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Texas Red® is a registered trademark of Molecular Probes, Inc.
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