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  • Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-FITC

Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-FITC

Cat. No.:
1083-02
Goat Anti-Mouse IgG2a-FITC antibody with minimal reactivity to human, bovine, and horse serum proteins for use in fluorescent assays.
$249.00
Size Price (USD) Quantity
1.0 mg $249.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with the heavy chain of mouse IgG2a
Source Pooled antisera from goats hyperimmunized with mouse IgG2a
Cross Adsorption Mouse IgG1, IgG2b, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2a covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2794507
Gene ID 380793 (Mouse)
Gene ID Symbol Ighg2a (Mouse)
Gene ID Aliases 1810060O09Rik; Igh-1; Igh-1a
UniProt ID P01863 (Mouse
P01864 (Mouse
P01865 (Mouse
UniProt Name GCAA_MOUSE (Mouse)
GCAB_MOUSE (Mouse)
GCAM_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with serially diluted Mouse IgG<sub>2a</sub>-UNLB (SB Cat. No. 0103-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgG2a-UNLB (HOPC-1)
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>-FITC (SB Cat. No. 1081-02).
    Goat Anti-Mouse IgG2a-FITC
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  4. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>2a</sub>, Human ads-FITC (SB Cat. No. 1082-02).
    Goat F(ab')2 Anti-Mouse IgG2a, Human ads-FITC
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  6. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-AF647 (SB Cat. No. 1090-31).
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  7. Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
    Goat IgG-FITC
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  8. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-AF488 (SB Cat. No. 1100-30).
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  9. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-FITC (SB Cat. No. 1080-02).
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  10. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>2a</sub>-FITC (SB Cat. No. 1155-02).
    Rat Anti-Mouse IgG2a-FITC (SB84a)
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  11. Wdpks1Δ-1 yeast cells treated with liquid nitrogen were stained with anti-melanin followed by Goat Anti-Mouse IgM, Human ads-TRITC (SB Cat. No. 1020-03).<br/>Image from Paolo WF Jr, Dadachova E, Mandal P, Casadevall A, Szaniszlo PJ, Nosanchuk JD. Effects of disrupting the polyketide synthase gene <i>WdPKS1</i> in <i>Wangiella</i> [<i>Exophiala</i>] <i>dermatitidis</i> on melanin production and resistance to killing by antifungal compounds, enzymatic degradation, and extremes in temperature. BMC Microbiol. 2006;6:55. Figure 5(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
    Goat Anti-Mouse IgM, Human ads-TRITC
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  12. Frozen section of TEPC 1165 cell line pellet was stained with Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07).<br/>Image from Wang H, Owens JD, Shih JH, Li M, Bonner RF, Mushinski JF. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics. 2006;7:97. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  13. BALB/c mouse splenocytes were stained with Goat Anti-Mouse Lambda-PE (SB Cat. No. 1060-09) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
    Goat Anti-Mouse Lambda-PE
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  14. FLISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-FITC (SB Cat. No. 1110-02).
    Goat Anti-Mouse IgE-FITC
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