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  • Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-BIOT

Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-BIOT

Cat. No.:
1083-08
Goat Anti-Mouse IgG2a-Biotin antibody with minimal reactivity to human, bovine, and horse serum proteins for use in ELISA assays.
$249.00
Size Price (USD) Quantity
1.0 mg $249.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy chain of mouse IgG2a
Source Pooled antisera from goats hyperimmunized with mouse IgG2a
Cross Adsorption Mouse IgG1, IgG2b, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2a covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2794510
Gene ID 380793 (Mouse)
Gene ID Symbol Ighg2a (Mouse)
Gene ID Aliases 1810060O09Rik; Igh-1; Igh-1a
UniProt ID P01863 (Mouse)
P01864 (Mouse)
P01865 (Mouse)
UniProt Name GCAA_MOUSE (Mouse)
GCAB_MOUSE (Mouse)
GCAM_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with serially diluted Mouse IgG<sub>2a</sub>-UNLB (SB Cat. No. 0103-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgG2a-UNLB (HOPC-1)
    0103-01
    $284.00
  2. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Ig, Human ads-UNLB (SB Cat. No. 1010-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Mouse Ig, Human ads-UNLB
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
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  4. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>-BIOT (SB Cat. No. 1081-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2a-BIOT
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  5. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  6. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG1, Human ads-BIOT
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  7. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
    Mouse Anti-Biotin-FITC (SB58c)
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  8. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1082-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Mouse IgG2a, Human ads-BIOT
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  9. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
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  10. ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgM, Human ads-BIOT
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  11. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2b, Human ads-BIOT
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  12. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  13. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG3, Human ads-BIOT
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  14. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2a, Human ads-BIOT
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  15. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>2a</sub>-BIOT (SB Cat. No. 1155-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Rat Anti-Mouse IgG2a-BIOT (SB84a)
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  16. ELISA plate was coated with purified mouse IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgA-BIOT
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  17. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-BIOT (SB Cat. No. 1079-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  18. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Kappa-BIOT (SB Cat. No. 1050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  19. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-BIOT (SB Cat. No. 1060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  20. ELISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-BIOT (SB Cat. No. 1110-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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