ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-BIOT (SB Cat. No. 1083-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).