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Mouse Anti-GSK-3α-HRP

Cat. No.
10905-05  
Size
1.0 mL  
Price (USD)
$360.00 
Qty
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Clone SBGSK3α2
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-HRP
Immunogen Recombinant N-terminal GSK-3α
Specificity Human/Mouse GSK-3α
Alternalte Name(s) Glycogen synthase kinase-3 alpha
Description Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3α and GSK-3β. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics, and cell motility.
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
Immunoblotting – Quality tested 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • GSK3α was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3α-UNLB (SB Cat. No. 10910-01). Total cell lysates from Jurkat cells (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3α-HRP (SB Cat. No. 10905-05). Proteins were visualized using chemiluminescent detection.

GSK3α was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3α-UNLB (SB Cat. No. 10910-01). Total cell lysates from Jurkat cells (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3α-HRP (SB Cat. No. 10905-05). Proteins were visualized using chemiluminescent detection.
 
References
1. SouthernBiotech published data
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