Goat Anti-Mouse IgG2b-AP

Cat. No.
1091-04  
Size
1.0 mL  
Price (USD)
$97.00 
Specificity Reacts with the heavy chain of mouse IgG2b
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Cross Adsorption Mouse IgG1, IgG2a, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG2b covalently linked to agarose
Isotype Goat IgG
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation Supplied in a stock solution of 50 mM Tris/1 mM MgCl2/50% glycerol, pH 8.0, containing NaN3 as a preservative
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b-AP (SB Cat. No. 1091-04).

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b-AP (SB Cat. No. 1091-04).
 
References
1. Silva-Sanchez A, Liu CR, Vale AM, Khass M, Kapoor P, Elgavish A, et al. Violation of an evolutionarily conserved immunoglobulin diversity gene sequence preference promotes production of dsDNA-specific IgG antibodies. PLoS One. 2015;10(2):e0118171. (ELISA)