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  • Goat Anti-Mouse IgG2b-BIOT

Goat Anti-Mouse IgG2b-BIOT

Cat. No.:
1091-08
Goat Anti-Mouse IgG2b-Biotin antibody for use in ELISA assays.
$203.00
Size Price (USD) Quantity
1.0 mg $203.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy chain of mouse IgG2b
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Cross Adsorption Mouse IgG1, IgG2a, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2b covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA

RRID Number AB_2794542
Gene ID 16016 (Mouse)
Gene ID Symbol Ighg2b (Mouse)
Gene ID Aliases IgG2b; Igh-3; gamma2b
UniProt ID P01867 (Mouse)
UniProt Name IGG2B_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Silva-Sanchez A, Liu CR, Vale AM, Khass M, Kapoor P, Elgavish A, et al. Violation of an evolutionarily conserved immunoglobulin diversity gene sequence preference promotes production of dsDNA-specific IgG antibodies. PLoS One. 2015;10(2):e0118171. (ELISA)

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  2. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Ig, Human ads-UNLB (SB Cat. No. 1010-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  6. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  8. Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) and Mouse Anti-Human CD44-FITC (SB Cat. No. 9400-02) followed by Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1092-08), Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
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  9. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  11. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  12. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  13. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  14. Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG<sub>2b</sub>, Human/Bovine/Horse SP ads-BIOT (SB Cat. No. 1093-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.
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  15. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Rat Anti-Mouse IgG<sub>2b</sub>-BIOT (SB Cat. No. 1186-08), Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
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  17. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-BIOT (SB Cat. No. 1079-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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