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Mouse Anti-GSK-3α-SEPH

Cat. No.
10910-25  
Size
50 tests  
Price (USD)
$425.00 
Qty
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Clone SBGSK3α4
Isotype Mouse (BALB/c) IgG2aκ
Immunogen Recombinant N-terminal GSK-3α
Specificity Human/Mouse GSK-3α
Alternalte Name(s) Glycogen synthase kinase-3 alpha
Description Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3α and GSK-3β. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics, and cell motility.
Format/Conjugate SEPH (Agarose)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Volume 2.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
Immunoblotting – Quality tested 3
Immunoprecipitation – Reported in literature 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • GSK3α was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3α-UNLB (SB Cat. No. 10910-01). Total cell lysates from Jurkat cells (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3α-HRP (SB Cat. No. 10905-05). Proteins were visualized using chemiluminescent detection.

GSK3α was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3α-UNLB (SB Cat. No. 10910-01). Total cell lysates from Jurkat cells (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3α-HRP (SB Cat. No. 10905-05). Proteins were visualized using chemiluminescent detection.
 
References
1. Meares GP, Zmijewska AA, Jope RS. HSP105 interacts with GRP78 and GSK3 and promotes ER stress-induced caspase-3 activation. Cell Signal. 2008;20:347-58. (IP)
2. Sun M, Song L, Li Y, Zhou T, Jope RS. Identification of an antiapoptotic protein complex at death receptors. Cell Death Differ. 2008;15:1887-1900. (IP)
3. SouthernBiotech published data
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