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Mouse Anti-Human GSK-3β-UNLB

Cat. No.
10915-01  
Size
0.1 mg  
Price (USD)
$315.00 
Qty
Add to Cart
Clone 5D6
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB
Immunogen Recombinant N-terminal GSK-3β
Specificity Human GSK-3β
Alternalte Name(s) Glycogen synthase kinase-3 beta
Description Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3α and GSK-3β. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics, and cell motility.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested
Immunoprecipitation – Quality tested 1,2
ELISA 3
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Total Jurkat cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3β-HRP (SB Cat. No. 10915-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05). Proteins were visualized using chemiluminescent detection.

Total Jurkat cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3β-HRP (SB Cat. No. 10915-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05). Proteins were visualized using chemiluminescent detection.
 
References
1. Meares GP, Zmijewska AA, Jope RS. HSP105 interacts with GRP78 and GSK3 and promotes ER stress-induced caspase-3 activation. Cell Signal. 2008;20:347-58. (IP)
2. Sun M, Song L, Li Y, Zhou T, Jope RS. Identification of an antiapoptotic protein complex at death receptors. Cell Death Differ. 2008;15:1887-1900. (IP)
3. SouthernBiotech unpublished data (ELISA)
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