Mouse Anti-Human GSK-3β-HRP
Cat. No.
10920-05
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Size
1.0 mL
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Price (USD)
$360.00
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Clone |
3A11
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Isotype |
Mouse (BALB/c) IgG2aκ
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Isotype Control |
Mouse IgG2a-HRP
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Immunogen |
Recombinant N-terminal GSK-3β
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Specificity |
Human GSK-3β
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Alternalte Name(s) |
Glycogen synthase kinase-3 beta
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Description |
Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3α and GSK-3β. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics, and cell motility.
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Format/Conjugate |
HRP (Horseradish Peroxidase)
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Buffer Formulation |
50% Glycerol/50% Phosphate buffered saline, pH 7.4
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Volume |
1.0 mL
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Storage & Handling |
2-8°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - ELISA – Quality tested Immunoblotiing – Reported in literature 1
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2794549
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Gene ID |
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Gene Symbol |
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Uniprot ID |
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Uniprot Name |
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GSK3β was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3β-SEPH (SB Cat. No. 10915-25). Immunoprecipitate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3β-HRP (SB Cat. No. 10920-05). Proteins were visualized using chemiluminescent detection.
GSK3β was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3β-SEPH (SB Cat. No. 10915-25). Immunoprecipitate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3β-HRP (SB Cat. No. 10920-05). Proteins were visualized using chemiluminescent detection.
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References
1. | Sun M, Song L, Li Y, Zhou T, Jope RS. Identification of an antiapoptotic protein complex at death receptors. Cell Death Differ. 2008;15:1887-1900. (WB) |
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