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Mouse Anti-Human GSK-3β-HRP

Cat. No.
10920-05  
Size
1.0 mL  
Price (USD)
$360.00 
Qty
Add to Cart
Clone 3A11
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-HRP
Immunogen Recombinant N-terminal GSK-3β
Specificity Human GSK-3β
Alternalte Name(s) Glycogen synthase kinase-3 beta
Description Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3α and GSK-3β. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics, and cell motility.
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Immunoblotiing – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • GSK3β was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3β-SEPH (SB Cat. No. 10915-25). Immunoprecipitate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3β-HRP (SB Cat. No. 10920-05). Proteins were visualized using chemiluminescent detection.

GSK3β was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3β-SEPH (SB Cat. No. 10915-25). Immunoprecipitate was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3β-HRP (SB Cat. No. 10920-05). Proteins were visualized using chemiluminescent detection.
 
References
1. Sun M, Song L, Li Y, Zhou T, Jope RS. Identification of an antiapoptotic protein complex at death receptors. Cell Death Differ. 2008;15:1887-1900. (WB)
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