Mouse Anti-Human FADD-HRP
Cat. No.
10941-05
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Size
1.0 mL
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Price (USD)
$305.00
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| Clone |
1A11
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| Isotype |
Mouse (BALB/c) IgG1κ
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| Isotype Control |
Mouse IgG1-HRP
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| Immunogen |
Recombinant FADD
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| Specificity |
Human FADD
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| Alternalte Name(s) |
FAS-associated death domain protein, GIG3, MGC8528, MORT1
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| Description |
Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1β-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.
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| Format/Conjugate |
HRP (Horseradish Peroxidase)
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| Buffer Formulation |
50% Glycerol/50% Phosphate buffered saline, pH 7.4
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| Volume |
1.0 mL
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| Storage & Handling |
2-8°C
Please refer to product specific SDS
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| Applications |
Applications for relevant formats of this clone include - ELISA – Quality tested
Western Blot – Quality tested
Immunoprecipitation – Quality tested
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| Recommended Dilutions |
Please refer to product specific Technical Bulletin
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| RRID |
AB_2794560
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| Gene ID |
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| Gene Symbol |
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| Gene Aliases |
GIG3; MORT1
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| Uniprot ID |
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| Uniprot Name |
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Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB (SB Cat. No. 10941-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB (SB Cat. No. 10941-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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