Mouse Anti-Human Bid-UNLB
Cat. No.
10951-01
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Size
0.1 mg
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Price (USD)
$225.00
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| Clone |
SB50a
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| Isotype |
Mouse (BALB/c) IgG2bκ
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| Isotype Control |
Mouse IgG2b-UNLB
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| Immunogen |
Recombinant Bid
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| Specificity |
Human Bid
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| Alternalte Name(s) |
Apoptic death agonist, BH3 domain-only protein, BH3 interacting domain death agonist
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| Description |
Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF-α apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c.
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| Format/Conjugate |
UNLB (Unconjugated)
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| Buffer Formulation |
Borate buffered saline, pH 8.2
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| Concentration |
0.5 mg/mL
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| Volume |
0.2 mL
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| Storage & Handling |
2-8°C
Please refer to product specific SDS
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| Applications |
Applications for relevant formats of this clone include - ELISA – Quality tested
Western Blot – Quality tested
Immunoprecipitation 1
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| Recommended Dilutions |
Please refer to product specific Technical Bulletin
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| RRID |
AB_2794562
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| Gene ID |
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| Gene Symbol |
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| Gene Aliases |
FP497
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| Uniprot ID |
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| Uniprot Name |
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Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bid-UNLB (SB Cat. No. 10951-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bid-UNLB (SB Cat. No. 10951-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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References
| 1. | SouthernBiotech unpublished data |
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