Mouse Anti-Human Bid-UNLB

Cat. No.
10951-01  
Size
0.1 mg  
Price (USD)
$225.00 
Clone SB50a
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB
Immunogen Recombinant Bid
Specificity Human Bid
Alternalte Name(s) Apoptic death agonist, BH3 domain-only protein, BH3 interacting domain death agonist
Description Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF-α apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Western Blot – Quality tested
Immunoprecipitation 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794562
Gene ID
637 (Human)
Gene Symbol
BID (Human)
Gene Aliases FP497
Uniprot ID
P55957 (Human)
Uniprot Name
  • Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bid-UNLB (SB Cat. No. 10951-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bid-UNLB (SB Cat. No. 10951-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 
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References
1. SouthernBiotech unpublished data