Rat Anti-Mouse IgM-HRP

Cat. No.
1139-05  
Size
1.0 mL  
Price (USD)
$195.00 
Clone SB73a
Isotype Rat (Sprague Dawley) IgG1κ
Isotype Control Rat IgG1-HRP
Immunogen Mouse IgM hybridoma
Specificity Mouse IgM
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested
ELISA – Quality tested
Western Blot 1
Immunohistochemistry-Paraffin Sections – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794625
Gene ID
16019 (Mouse)
Gene Symbol
Ighm (Mouse)
Gene Aliases AI326478; Igh-6; Igh-M; Igh6; Igm; muH
Uniprot ID
P01872 (Mouse)
Uniprot Name
  • ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-HRP (SB Cat. No. 1139-05).
  • Paraffin embedded scrapie-infected sheep retropharyngeal lymph node section was stained with anti-120 kDa followed by Rat Anti-Mouse IgM-HRP (SB Cat. No. 1139-05) and DAB along with anti-prion protein followed by secondary reagents and Fast Red.

    Image from Dassanayake RP, Truscott TC, Özyiğit MÖ, Zhuang D, Schneider DA, O'Rourke KI. Accumulation profiles of PrPSc in hemal nodes of naturally and experimentally scrapie-infected sheep. BMC Vet Res. 2013;9:82. Figure 3(g)

ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-HRP (SB Cat. No. 1139-05).
 

Paraffin embedded scrapie-infected sheep retropharyngeal lymph node section was stained with anti-120 kDa followed by Rat Anti-Mouse IgM-HRP (SB Cat. No. 1139-05) and DAB along with anti-prion protein followed by secondary reagents and Fast Red.

Image from Dassanayake RP, Truscott TC, Özyiğit MÖ, Zhuang D, Schneider DA, O'Rourke KI. Accumulation profiles of PrPSc in hemal nodes of naturally and experimentally scrapie-infected sheep. BMC Vet Res. 2013;9:82. Figure 3(g)
 
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References
1. SouthernBiotech unpublished data (WB)
2. Dassanayake RP, Truscott TC, Özyiğit MÖ, Zhuang D, Schneider DA, O'Rourke KI. Accumulation profiles of PrPSc in hemal nodes of naturally and experimentally scrapie-infected sheep. BMC Vet Res. 2013;9:82. (IHC-PS)