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  • Rat Anti-Mouse IgM-UNLB (SB73a)

Rat Anti-Mouse IgM-UNLB (SB73a)

Cat. No.:
1139-01
Purified Anti-Mouse IgM antibody for use in ELISA, flow cytometry, immunohistochemistry, and western blot assays.
$174.00
Size Price (USD) Quantity
0.5 mg $174.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB73a
Isotype Rat (Sprague Dawley) IgG1κ
Isotype Control Rat IgG1-UNLB (KLH/G1-2-2)
Specificity Mouse IgM
Immunogen Mouse IgM hybridoma
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested
ELISA – Quality tested
Western Blot 1
Immunohistochemistry-Paraffin Sections – Reported in literature 2

RRID Number AB_2794623
Gene ID 16019 (Mouse)
Gene ID Symbol Ighm (Mouse)
Gene ID Aliases AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID P01872 (Mouse)
UniProt Name IGHM_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. SouthernBiotech unpublished data (WB)
  2. 2. Dassanayake RP, Truscott TC, Özyiğit MÖ, Zhuang D, Schneider DA, O'Rourke KI. Accumulation profiles of PrPSc in hemal nodes of naturally and experimentally scrapie-infected sheep. BMC Vet Res. 2013;9:82. (IHC-PS)

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  4. Paraffin embedded mouse small intestine section was stained with Rat Anti-Mouse IgE-UNLB (SB Cat. No. 1130-01; right) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin.
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  5. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  7. FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>1</sub>-FITC (SB Cat. No. 3060-02).
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  9. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  11. Paraffin embedded BALB/c mouse colon tissue section was stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-FITC (SB Cat. No. 6420-02) and DAPI.
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  23. Supernatant fractions from Bax-/- mouse embryonic fibroblasts were incubated with caspase-8-cleaved human Bid (tBid) and anti-Bak (Clone 7D10 and Clone 8F8), resolved by electrophoresis, transferred to membrane, and probed with anti-cytochrome c followed by Goat Anti-Mouse Ig, Human ads-HRP (SB Cat. No. 1010-05) and chemiluminescent detection.<br/>Image from Iyer S, Anwari K, Alsop AE, Yuen WS, Huang DC, Carroll J, et al. Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nat Commun. 2016;7:11734. Figure 1(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  24. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-UNLB (SB Cat. No. 1079-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  25. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-SPRD (SB Cat. No. 3052-13).
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