Rat Anti-Mouse IgG2b-BIOT

Cat. No.
1186-08  
Size
0.5 mg  
Price (USD)
$190.00 
Clone SB74g
Isotype Rat (Sprague Dawley) IgG2bλ
Isotype Control Rat IgG2b-BIOT
Immunogen Mouse IgG2b hybridoma
Specificity Mouse IgG2b
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested
ELISA – Quality tested 1-3
FLISA – Quality tested
Immunocytochemistry 5
Western Blot 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794688
Gene ID
16016 (Mouse)
Gene Symbol
Ighg2b (Mouse)
Gene Aliases IgG2b; Igh-3; gamma2b
Uniprot ID
P01867 (Mouse)
Uniprot Name
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Rat Anti-Mouse IgG2b-BIOT (SB Cat. No. 1186-08), Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
  • ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG2b-BIOT (SB Cat. No. 1186-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • ELISA plate was coated with mouse serum and rat serum. Sera were detected with serially diluted Rat Anti-Mouse IgG2b-BIOT (SB Cat. No. 1186-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Rat Anti-Mouse IgG2b-BIOT (SB Cat. No. 1186-08), Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
 

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG2b-BIOT (SB Cat. No. 1186-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

ELISA plate was coated with mouse serum and rat serum. Sera were detected with serially diluted Rat Anti-Mouse IgG2b-BIOT (SB Cat. No. 1186-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Chen M, Hong MJ, Sun H, Wang L, Shi X, Gilbert BE, et al. Essential role for autophagy in the maintenance of immunological memory against influenza infection. Nat Med. 2014;20:503-10. (ELISA)
2. de Gouw D, Serra DO, de Jonge MI, Hermans PW, Wessels HJ, Zomer A, et al. The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins. Emerg Microbes Infect. 2014;3:e58. (ELISA)
3. Zuest R, Valdes I, Skibinski D, Lin Y, Toh YX, Chan K, et al. Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice. Vaccine. 2015;33:1474-82. (ELISA)
4. SouthernBiotech unpublished data (WB)
5. SouthernBiotech published data (ICC)