Bovine Type I Collagen-Lyophilized

Cat. No.
1200-02  
Size
0.25 mg  
Price (USD)
$150.00 
Description Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type I collagen is the most abundant collagen and is expressed in almost all connective tissues including skin, tendon, and bone tissue. It is also the predominant component of the interstitial matrix. Type I collagen mutations are associated in a range of diseases including osteogenesis imperfecta and Ehlers–Danlos syndrome. Type I collagen consists of two α1(I) chains and one α2(I) chain.
Source Placental villi
Purity > 90% by SDS-PAGE
Purification Controlled and limited pepsin digestion followed by selective salt precipitation
Alternalte Name(s) COL1A1, COL1A2
Buffer Formulation Lyophilized from 500 mM acetic acid
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested
SDS-PAGE – Quality tested
Surface Plasmon Resonance – Reported in literature 2
Coating Material for –
Adhesion Studies – Reported in literature 1-3
Migration Studies – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Bovine Type I Collagen (SB Cat. No. 1200-02). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with serially diluted Bovine Type I Collagen (SB Cat. No. 1200-02). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Switalski LM, Butcher WG, Caufield PC, Lantz MS. Collagen mediates adhesion of Streptococcus mutans to human dentin. Infect Immun. 1993;61:4119-25. (Coating, Adhesion Studies)
2. House-Pompeo K, Boles JO, Höök M. Characterization of bacterial adhesin interactions with extracellular matrix components utilizing biosensor technology. Methods. 1994;6:134-42. (SPR, Coating, Adhesion Studies)
3. McGrady JA, Butcher WG, Beighton D, Switalski LM. Specific and charge interactions mediate collagen recognition by oral lactobacilli. J Dent Res. 1995;74:649-57. (Coating, Adhesion Studies)
4. Augustin-Voss HG, Pauli BU. Quantitative analysis of autocrine-regulated, matrix-induced, and tumor cell-stimulated endothelial cell migration using a silicon template compartmentalization technique. Exp Cell Res. 1992;198:221-7. (Coating, Migration Studies)