Rat Type I Collagen-Lyophilized

Cat. No.
1200-03  
Size
0.25 mg  
Price (USD)
$150.00 
Description Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type I collagen is the most abundant collagen and is expressed in almost all connective tissues including skin, tendon, and bone tissue. It is also the predominant component of the interstitial matrix. Type I collagen mutations are associated in a range of diseases including osteogenesis imperfecta and Ehlers–Danlos syndrome. Type I collagen consists of two α1(I) chains and one α2(I) chain.
Source Tail tendon
Purity > 90% by SDS-PAGE
Purification Selective salt precipitation
Alternalte Name(s) Col1a1, Col1a2
Buffer Formulation Lyophilized from 500 mM acetic acid
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested
SDS-PAGE – Quality tested
Adhesion Studies – Reported in literature 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Rat Type I Collagen (SB Cat. No. 1200-03). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with serially diluted Rat Type I Collagen (SB Cat. No. 1200-03). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Leonard CA. Microsporidia spore adherence and host cell infection in vitro [dissertation]. Johnson City (USA): East Tennessee State University; 2013. (Adhesion Studies)
2. Leonard CA, Hayman JR. Role of host cell integrins in the microsporidium Encephalitozoon intestinalis adherence and infection in vitro. FEMS Microbiol Lett. 2017;364:fnx169. (Adhesion Studies)