Mouse Anti-Human Erk1/2-BIOT

Cat. No.
12080-08  
Size
0.5 mg  
Price (USD)
$204.00 
Clone SB46b
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen Recombinant human Erk1 and Erk2
Specificity Human Erk1 and human Erk2
Alternalte Name(s) MAPK, p44/42
Description Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens, and differentiation-promoting agents via a protein kinase cascade. Erk1 and Erk2 are ubiquitous and abundant although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding. Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. Both sites must be phosphorylated for maximum activity. These kinases in turn phosphorylate a variety of different substrates.

Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. They target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus.

The SB46b monoclonal antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested
ELISA 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794717
Gene ID
5595 (Human)
5594 (Human)
Gene Symbol
MAPK3 (Human)
MAPK1 (Human)
Gene Aliases ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK; ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK
Uniprot ID
P27361 (Human)
P28482 (Human)
Uniprot Name
  • Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.

Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (SB Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05) secondary antibody and chemiluminescent detection.
 
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References
1. SouthernBiotech unpublished data (ELISA)