Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB

Cat. No.
13200-01  
Size
0.1 mg  
Price (USD)
$265.00 
Clone SB146a
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen Synthetic peptide designed based on the region around Lysine 9 of human H2A sequence
Specificity Human H2A acetylated at Lysine 9
Alternalte Name(s) H2AK9ac
Description Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Western Blot 1
Immunoprecipitation 1
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Flow Cytometry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794729
  • Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-BIOT (SB Cat. No. 0103-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H2A (Lys9) (SB Cat No. 13200) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
  • H2AK9ac was immunoprecipitated from total cell lysates from Jurkat cells treated with trichostatin A (TSA) using Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (Cat. No. 13200-01). Total cell lysates from Jurkat cells treated with trichostatin A (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (Cat. No. 13200-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.
  • Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-PE (SB Cat. No. 1030-09).
  • Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
  • Lane 1 - Untreated
    Lane 2 - 1,000 pM TSA-treated
    Lane 3 - 200 pM TSA-treated
    Lane 4 - 40 pM TSA-treated
    Lane 5 - 8 pM TSA-treated
    Jurkat cell lysates were treated in a dose dependent manner with trichostatin A (TSA), resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
  • Trichostatin A (TSA) treated and untreated human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-BIOT (SB Cat. No. 0103-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H2A (Lys9) (SB Cat No. 13200) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-BIOT (SB Cat. No. 0103-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H2A (Lys9) (SB Cat No. 13200) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
 

H2AK9ac was immunoprecipitated from total cell lysates from Jurkat cells treated with trichostatin A (TSA) using Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (Cat. No. 13200-01). Total cell lysates from Jurkat cells treated with trichostatin A (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (Cat. No. 13200-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05) secondary antibody and chemiluminescent detection.
 

Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-PE (SB Cat. No. 1030-09).
 

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 

Lane 1 - Untreated
Lane 2 - 1,000 pM TSA-treated
Lane 3 - 200 pM TSA-treated
Lane 4 - 40 pM TSA-treated
Lane 5 - 8 pM TSA-treated
Jurkat cell lysates were treated in a dose dependent manner with trichostatin A (TSA), resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H2A (Lys9)-UNLB (SB Cat. No. 13200-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 

Trichostatin A (TSA) treated and untreated human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-BIOT (SB Cat. No. 0103-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H2A (Lys9) (SB Cat No. 13200) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
 
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References
1. SouthernBiotech published data