Mouse Anti-Acetyl-Histone H3 (Lys18)-UNLB

Cat. No.
13300-01  
Size
0.1 mg  
Price (USD)
$265.00 
Clone SB157a
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Synthetic peptide designed based on the region around Lysine 18 of human H3 sequence
Specificity Human H3 acetylated at Lysine 18
Alternalte Name(s) H3K18ac
Description Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Western Blot 1
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Flow Cytometry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794733
  • Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H3 (Lys18) (SB Cat No. 13300) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
  • Lane 1 - Untreated
    Lane 2 - 1,000 pM TSA-treated
    Lane 3 - 200 pM TSA-treated
    Lane 4 - 40 pM TSA-treated
    Lane 5 - 8 pM TSA-treated
    Lane 6 - 1.6 pM TSA-treated
    Jurkat cell lysates were treated in a dose dependent manner with trichostatin A (TSA), resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H3(Lys18)-UNLB (SB Cat. No. 13300-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
  • Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H3 (Lys18)-UNLB (SB Cat. No. 13300-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
  • Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H3 (Lys18)-UNLB (SB Cat. No. 13300-01) followed by Goat Anti-Mouse IgG, Human ads-PE (SB Cat. No. 1030-09).

Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H3 (Lys18) (SB Cat No. 13300) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
 

Lane 1 - Untreated
Lane 2 - 1,000 pM TSA-treated
Lane 3 - 200 pM TSA-treated
Lane 4 - 40 pM TSA-treated
Lane 5 - 8 pM TSA-treated
Lane 6 - 1.6 pM TSA-treated
Jurkat cell lysates were treated in a dose dependent manner with trichostatin A (TSA), resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H3(Lys18)-UNLB (SB Cat. No. 13300-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H3 (Lys18)-UNLB (SB Cat. No. 13300-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 

Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H3 (Lys18)-UNLB (SB Cat. No. 13300-01) followed by Goat Anti-Mouse IgG, Human ads-PE (SB Cat. No. 1030-09).
 
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References
1. SouthernBiotech published data