Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB
Cat. No.
13400-01
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Size
0.1 mg
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Price (USD)
$265.00
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Clone |
SB159b
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Isotype |
Mouse (BALB/c) IgG1κ
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Isotype Control |
Mouse IgG1-UNLB
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Immunogen |
Synthetic peptide designed based on the region around Lysine 12 of human H4 sequence
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Specificity |
Human H4 acetylated at Lysine 12
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Alternalte Name(s) |
H4K12ac
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Description |
Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
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Format/Conjugate |
UNLB (Unconjugated)
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Buffer Formulation |
Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
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Concentration |
0.5 mg/mL
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Volume |
0.2 mL
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Storage & Handling |
-20°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - ELISA – Quality tested Western Blot 1 Immunoprecipitation 2 Immunohistochemistry-Paraffin Sections 1 Immunocytochemistry 1 Flow Cytometry 2
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2794736
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Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.
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Lane 1 - 30 mM Curcumin-treated Lane 2 - 15 mM Curcumin-treated Lane 3 - 7.5 mM Curcumin-treated Lane 4 - Untreated Jurkat cell lysates were treated in a dose dependent manner with the acetyltransferase inhibitor curcumin, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-b-Actin-HRP and Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H4 (Lys12) (SB Cat No. 13400) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.
Lane 1 - 30 mM Curcumin-treated Lane 2 - 15 mM Curcumin-treated Lane 3 - 7.5 mM Curcumin-treated Lane 4 - Untreated Jurkat cell lysates were treated in a dose dependent manner with the acetyltransferase inhibitor curcumin, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-b-Actin-HRP and Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H4 (Lys12) (SB Cat No. 13400) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
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References
1. | SouthernBiotech published data | 2. | SouthernBiotech unpublished data |
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