Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB

Cat. No.
13400-01  
Size
0.1 mg  
Price (USD)
$265.00 
Clone SB159b
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Synthetic peptide designed based on the region around Lysine 12 of human H4 sequence
Specificity Human H4 acetylated at Lysine 12
Alternalte Name(s) H4K12ac
Description Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Western Blot 1
Immunoprecipitation 2
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Flow Cytometry 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794736
  • Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.
  • Lane 1 - 30 mM Curcumin-treated
    Lane 2 - 15 mM Curcumin-treated
    Lane 3 - 7.5 mM Curcumin-treated
    Lane 4 - Untreated
    Jurkat cell lysates were treated in a dose dependent manner with the acetyltransferase inhibitor curcumin, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-b-Actin-HRP and Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
  • Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H4 (Lys12) (SB Cat No. 13400) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.
 

Lane 1 - 30 mM Curcumin-treated
Lane 2 - 15 mM Curcumin-treated
Lane 3 - 7.5 mM Curcumin-treated
Lane 4 - Untreated
Jurkat cell lysates were treated in a dose dependent manner with the acetyltransferase inhibitor curcumin, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-b-Actin-HRP and Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 

Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H4 (Lys12) (SB Cat No. 13400) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
 
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References
1. SouthernBiotech published data
2. SouthernBiotech unpublished data