Unlabeled Anti-Acetyl-Histone H4 (Lys12), SB159b

Cat. No.
13400-01

Size
0.1 mg

Price (USD)
$265.00

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Clone	SB159b
Isotype	Mouse (BALB/c) IgG₁κ
Isotype Control	Mouse IgG₁-UNLB
Immunogen	Synthetic peptide designed based on the region around Lysine 12 of human H4 sequence
Specificity	Human H4 acetylated at Lysine 12
Alternalte Name(s)	H4K12ac
Description	Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration	0.5 mg/mL
Volume	0.2 mL
Storage & Handling	-20°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA – Quality tested Western Blot ¹ Immunoprecipitation ² Immunohistochemistry-Paraffin Sections ¹ Immunocytochemistry ¹ Flow Cytometry ²
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794736

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.
Lane 1 - 30 mM Curcumin-treated
Lane 2 - 15 mM Curcumin-treated
Lane 3 - 7.5 mM Curcumin-treated
Lane 4 - Untreated
Jurkat cell lysates were treated in a dose dependent manner with the acetyltransferase inhibitor curcumin, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-b-Actin-HRP and Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H4 (Lys12) (SB Cat No. 13400) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05), DAB, and hematoxylin.

Lane 1 - 30 mM Curcumin-treated
Lane 2 - 15 mM Curcumin-treated
Lane 3 - 7.5 mM Curcumin-treated
Lane 4 - Untreated
Jurkat cell lysates were treated in a dose dependent manner with the acetyltransferase inhibitor curcumin, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-b-Actin-HRP and Mouse Anti-Acetyl-Histone H4 (Lys12)-UNLB (SB Cat. No. 13400-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG1-BIOT (SB Cat. No. 0102-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl-Histone H4 (Lys12) (SB Cat No. 13400) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.