Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB
Cat. No.
13500-01
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Size
0.1 mg
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Price (USD)
$265.00
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Clone |
SB153a
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Isotype |
Mouse (BALB/c) IgG2bκ
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Isotype Control |
Mouse IgG2b-UNLB
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Immunogen |
Synthetic peptide designed based on the region around Threonine 3 of human H3 sequence
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Specificity |
Human/Mouse H3 phosphorylated at Threonine 3
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Alternalte Name(s) |
H3T3P
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Description |
Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
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Format/Conjugate |
UNLB (Unconjugated)
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Buffer Formulation |
Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
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Concentration |
0.5 mg/mL
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Volume |
0.2 mL
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Storage & Handling |
-20°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - ELISA – Quality tested Western Blot 1 Immunoprecipitation 2 Immunohistochemistry-Paraffin Sections 1 Immunocytochemistry 1 Flow Cytometry 1
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2794739
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Lane 1 - Untreated Lane 2 - CLA / OA-treated Calyculin-A (CLA) / okadaic acid (OA) treated and untreated HEp-2 cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.
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Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2b-BIOT (SB Cat. No. 0104-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat No. 13500) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
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Paraffin embedded mouse small intestine tissue was stained with Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to biotin followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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Calyculin-A (CLA) / okadaic acid (OA) treated and untreated human epithelial carcinoma cell line HEp-2 was intracellularly stained with Mouse Anti-Phosho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to FITC.
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Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
Lane 1 - Untreated Lane 2 - CLA / OA-treated Calyculin-A (CLA) / okadaic acid (OA) treated and untreated HEp-2 cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.
Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2b-BIOT (SB Cat. No. 0104-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat No. 13500) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
Paraffin embedded mouse small intestine tissue was stained with Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to biotin followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
Calyculin-A (CLA) / okadaic acid (OA) treated and untreated human epithelial carcinoma cell line HEp-2 was intracellularly stained with Mouse Anti-Phosho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to FITC.
Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
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References
1. | SouthernBiotech published data | 2. | SouthernBiotech unpublished data |
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