Unlabeled Anti-Phospho-Histone H3 (Thr3), SB153a

Cat. No.
13500-01

Size
0.1 mg

Price (USD)
$265.00

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Clone	SB153a
Isotype	Mouse (BALB/c) IgG_2bκ
Isotype Control	Mouse IgG_2b-UNLB
Immunogen	Synthetic peptide designed based on the region around Threonine 3 of human H3 sequence
Specificity	Human/Mouse H3 phosphorylated at Threonine 3
Alternalte Name(s)	H3T3P
Description	Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration	0.5 mg/mL
Volume	0.2 mL
Storage & Handling	-20°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - ELISA – Quality tested Western Blot ¹ Immunoprecipitation ² Immunohistochemistry-Paraffin Sections ¹ Immunocytochemistry ¹ Flow Cytometry ¹
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794739

Lane 1 - Untreated
Lane 2 - CLA / OA-treated
Calyculin-A (CLA) / okadaic acid (OA) treated and untreated HEp-2 cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.
Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2b-BIOT (SB Cat. No. 0104-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat No. 13500) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
Paraffin embedded mouse small intestine tissue was stained with Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to biotin followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
Calyculin-A (CLA) / okadaic acid (OA) treated and untreated human epithelial carcinoma cell line HEp-2 was intracellularly stained with Mouse Anti-Phosho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to FITC.
Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Lane 1 - Untreated
Lane 2 - CLA / OA-treated
Calyculin-A (CLA) / okadaic acid (OA) treated and untreated HEp-2 cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.

Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2b-BIOT (SB Cat. No. 0104-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat No. 13500) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.

Paraffin embedded mouse small intestine tissue was stained with Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to biotin followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.

Calyculin-A (CLA) / okadaic acid (OA) treated and untreated human epithelial carcinoma cell line HEp-2 was intracellularly stained with Mouse Anti-Phosho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to FITC.

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).