Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB

Cat. No.
13500-01  
Size
0.1 mg  
Price (USD)
$265.00 
Clone SB153a
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB
Immunogen Synthetic peptide designed based on the region around Threonine 3 of human H3 sequence
Specificity Human/Mouse H3 phosphorylated at Threonine 3
Alternalte Name(s) H3T3P
Description Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Western Blot 1
Immunoprecipitation 2
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 1
Flow Cytometry 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794739
  • Lane 1 - Untreated
    Lane 2 - CLA / OA-treated
    Calyculin-A (CLA) / okadaic acid (OA) treated and untreated HEp-2 cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.
  • Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2b-BIOT (SB Cat. No. 0104-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat No. 13500) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
  • Paraffin embedded mouse small intestine tissue was stained with Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to biotin followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
  • Calyculin-A (CLA) / okadaic acid (OA) treated and untreated human epithelial carcinoma cell line HEp-2 was intracellularly stained with Mouse Anti-Phosho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to FITC.
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Lane 1 - Untreated
Lane 2 - CLA / OA-treated
Calyculin-A (CLA) / okadaic acid (OA) treated and untreated HEp-2 cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.
 

Human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2b-BIOT (SB Cat. No. 0104-08; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat No. 13500) conjugated to biotin followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.
 

Paraffin embedded mouse small intestine tissue was stained with Mouse Anti-Phospho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to biotin followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 

Calyculin-A (CLA) / okadaic acid (OA) treated and untreated human epithelial carcinoma cell line HEp-2 was intracellularly stained with Mouse Anti-Phosho-Histone H3 (Thr3) (SB Cat. No. 13500) conjugated to FITC.
 

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
 
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References
1. SouthernBiotech published data
2. SouthernBiotech unpublished data