Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB

Cat. No.
13600-01  
Size
0.1 mg  
Price (USD)
$265.00 
Clone SB147d
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB
Immunogen Synthetic peptide designed based on the region around Lysine 4 of human H3 sequence
Specificity Human H3 acetylated at Lysine 4
Alternalte Name(s) H3K4ac
Description Nucleosomes are the fundamental repeating subunit of chromatin and are the basic units of DNA packaging in eukaryotes. Nucleosomes consist of 147 base pairs of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histones consist of a globular domain and a more flexible amino terminus (histone “tail”) which may undergo various post-translational modifications, including acetylation, phosphorylation, and methylation. These modifications have a direct effect on chromatin structure and chromatin protein interactions, and are involved in DNA repair, chromosome condensation, and gene regulation.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2, containing 30% glycerol and 0.01% BSA
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling -20°C
Please refer to product specific SDS
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794742
  • Trichostatin A (TSA) treated human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-UNLB (SB Cat. No. 0104-01; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl- Histone H3 (Lys4)-UNLB (SB Cat No. 13600-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
  • Lane 1 - Untreated Jurkat whole cell lysate
    Lane 2 - TSA-treated Jurkat whole cell lysate
    Trichostatin A (TSA) treated and untreated Jurkat cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1093-05) secondary antibody and chemiluminescent detection.
  • Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG, Human ads-APC (SB Cat. No. 1030-11).
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
  • Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
  • Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; IgG2a) and Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01; IgG2b) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04) and Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), Red AP, DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Trichostatin A (TSA) treated human pancreatic carcinoma cell line MIA PaCa-2 was intracellularly stained with Mouse IgG2a-UNLB (SB Cat. No. 0104-01; left), Mouse Anti-Cytokeratin 18-FITC (SB Cat. No. 10085-02), and Mouse Anti-Acetyl- Histone H3 (Lys4)-UNLB (SB Cat No. 13600-01) followed by Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
 

Lane 1 - Untreated Jurkat whole cell lysate
Lane 2 - TSA-treated Jurkat whole cell lysate
Trichostatin A (TSA) treated and untreated Jurkat cell lysates were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-HRP (SB Cat. No. 1093-05) secondary antibody and chemiluminescent detection.
 

Trichostatin A (TSA) treated and untreated human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG, Human ads-APC (SB Cat. No. 1030-11).
 

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
 

Paraffin embedded human gastric cancer tissue was stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; IgG2a) and Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01; IgG2b) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04) and Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), Red AP, DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).
 
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References
1. SouthernBiotech published data
2. SouthernBiotech unpublished data