Rat Anti-Mouse CD8α-APC

Cat. No.
1550-11  
Size
0.1 mg   		Price (USD)
$180.00 
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Clone 53-6.7
Isotype Rat (LOU/Ws1/M) IgG2aκ
Isotype Control Rat IgG2a-APC
Immunogen Spleen cells or thymocyte membranes
Specificity Mouse CD8α
Alternalte Name(s) Ly-2, Lyt-2, Ly-35, Ly-B, T8
Description In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Format/Conjugate APC (Allophycocyanin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,6,7,9-15
Immunohistochemistry-Frozen Sections – Reported in literature 2-5
Immunocytochemistry – Reported in literature 7
Immunoprecipitation – Reported in literature 1,6
Depletion – Reported in literature 8
Blocking – Reported in literature 6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794877
Gene ID
12525 (Mouse)
Gene Symbol
Cd8a (Mouse)
Gene Aliases Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Uniprot ID
P01731 (Mouse)
Uniprot Name
CD8A_MOUSE 
  • Rat Anti-Mouse CD8a-APC
    BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD8α-APC (SB Cat. No. 1550-11) and Rat Anti-Mouse CD4-FITC (SB Cat. No. 1540-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD8α-APC (SB Cat. No. 1550-11) and Rat Anti-Mouse CD4-FITC (SB Cat. No. 1540-02).
 
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References
1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, IP, FC)
2. Konno A, Takada K, Saegusa J, Takiguchi M. Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjörgren's syndrome. Autoimmunity. 2003;36:247-54. (IHC-FS)
3. Delong P, Tanaka T, Kruklitis R, Henry AC, Kapoor V, Kaiser LR, et al. Use of cyclooxygenase-2 inhibition to enhance the efficacy of immunotherapy. Cancer Res. 2003;63:7845-52. (IHC-FS)
4. Wehling-Henricks M, Jordan MC, Gotoh T, Grody WW, Roos KP, Tidball JG. Arginine metabolism by macrophages promotes cardiac and muscle fibrosis in mdx muscular dystrophy. PloS One. 2010;5(5):e10763. (IHC-FS)
5. Kunikata N, Sano K, Honda M, Ishii K, Matsunaga J, Okuyama R, et al. Peritumoral CpG oligodeoxynucleotide treatment inhibits tumor growth and metastasis of B16F10 melanoma cells. J Invest Dermatol. 2004;123:395-402. (IHC-FS)
6. Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981;153:1503-16. (IP, FC, Block)
7. Choi J, Oh S, Lee D, Oh HJ, Park JY, Lee SB, et al. Mst1-FoxO signaling protects naïve T lymphocytes from cellular oxidative stress in mice. PloS One. 2009;4(11):e8011. (FC, ICC)
8. Hathcock KS. T cell depletion by cytotoxic elimination. Curr Protoc Immunol. 1991;3:4.1. (Depletion)
9. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (FC)
10. Parmo-Cabañas M, García-Bernal D, García-Verdugo R, Kremer L, Márquez G, Teixidó J. Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin α4β1. J Leukoc Biol. 2007;82:380-91. (FC)
11. Fink LN, Frøkiær H. Dendritic cells from Peyer's patches and mesenteric lymph nodes differ from spleen dendritic cells in their response to commensal gut bacteria. Scand J Immunol. 2008;68:270-9. (FC)
12. Jordan KR, Buhrman JD, Sprague J, Moore BL, Gao D, Kappler JW, et al. TCR hypervariable regions expressed by T cells that respond to effective tumor vaccines. Cancer Immunol Immunother. 2012;61:1627-38. (FC)
13. Elgbratt K, Jansson A, Hultgren-Hörnquist E. A quantitative study of the mechanisms behind thymic atrophy in Gαi2-deficient mice during colitis development. PLoS One. 2012;7(5):e36726. (FC)
14. Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC)
15. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)