Mouse Anti-Mouse CD19-UNLB

Cat. No.
1576-01  
Size
0.5 mg  
Price (USD)
$197.00 
Clone MB19-1
Isotype Mouse (129 x C57BL/6) IgAκ
Isotype Control Mouse IgA-UNLB
Immunogen CD19+ mouse pre-B cell line 300.19
Specificity Mouse CD19
Alternalte Name(s) B4, Leu-12
Description CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,6
Immunoprecipitation – Reported in literature 1-3,7
Activation – Reported in literature 4,5,7
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794963
Gene ID
12478 (Mouse)
Gene Symbol
Cd19 (Mouse)
Gene Aliases AW495831
Uniprot ID
P25918 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD19-UNLB (SB Cat. No. 1576-01) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09) followed by Goat Anti-Mouse IgA-FITC (SB Cat. No. 1040-02).
  • BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD19-UNLB (SB Cat. No. 1576-01) followed by Goat Anti-Mouse IgA-FITC (SB Cat. No. 1040-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD19-UNLB (SB Cat. No. 1576-01) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09) followed by Goat Anti-Mouse IgA-FITC (SB Cat. No. 1040-02).
 

BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD19-UNLB (SB Cat. No. 1576-01) followed by Goat Anti-Mouse IgA-FITC (SB Cat. No. 1040-02).
 
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References
1. Sato S, Ono N, Steeber DA, Pisetsky DS, Tedder TF. CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol. 1996;157:4371-8. (Immunogen, FC, IP)
2. Yazawa N, Fujimoto M, Sato S, Miyake K, Asano N, Nagai Y, et al. CD19 regulates innate immunity by the toll-like receptor RP105 signaling in B lymphocytes. Blood. 2003;102:1374-80. (IP)
3. Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF. CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999;162:7088-94. (IP)
4. Fujimoto M, Poe JC, Hasegawa M, Tedder TF. CD19 amplification of B lymphocyte Ca2+ responses: a role for Lyn sequestration in extinguishing negative regulation. J Biol Chem. 2001;276:44820-7. (Activ)
5. Sato S, Jansen PJ, Tedder TF. CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling. Proc Natl Acad Sci USA. 1997;94:13158-62. (Activ)
6. Sato S, Steeber DA, Jansen PJ, Tedder TF. CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19. J Immunol. 1997;158:4662-9. (FC)
7. Lee Y, Haas KM, Gor DO, Ding X, Karp DR, Greenspan NS, et al. Complement component C3d-antigen complexes can either augment or inhibit B lymphocyte activation and humoral immunity in mice depending on the degree of CD21/CD19 complex engagement. J Immunol. 2005;175:8011-23. (FC, IP, Activ)