Rat Anti-Human Caspase-2-UNLB
Cat. No.
17100-01
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Size
0.1 mg
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Price (USD)
$200.00
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| Clone |
SB123a
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| Isotype |
Rat (Sprague Dawley) IgMκ
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| Isotype Control |
Rat IgM-UNLB
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| Immunogen |
Purified native human caspase-2
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| Specificity |
Human caspase-2
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| Alternalte Name(s) |
Nedd2, ICH-1, CASP2
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| Description |
The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase-2 is an initiator caspase that is prevalent in both the cytosol and nucleus in many cell types. During embryonic development it is expressed at relatively high levels in the central nervous system, liver, kidney, and lungs and is prevalent in numerous tissues in adults. Studies have shown that caspase-2 becomes activated in response to several lethal stimuli including DNA damage, ER stress, and death receptor stimulation and further evidence has suggested a role in reactive oxygen species-induced cell death.
The SB123a monoclonal antibody detects bands at ~40 and ~48 kDa corresponding to cleaved and uncleaved forms of caspase-2, respectively.
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| Format/Conjugate |
UNLB (Unconjugated)
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| Buffer Formulation |
Borate buffered saline, pH 8.2
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| Concentration |
1.0 mg/mL
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| Volume |
0.1 mL
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| Storage & Handling |
2-8°C
Please refer to product specific SDS
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| Applications |
Applications for relevant formats of this clone include - Western Blot – Quality tested
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| Recommended Dilutions |
Please refer to product specific Technical Bulletin
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| RRID |
AB_2795175
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| Gene ID |
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| Gene Symbol |
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| Gene Aliases |
CASP-2; ICH1; NEDD-2; NEDD2; PPP1R57
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| Uniprot ID |
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| Uniprot Name |
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Total cell lysates from Jurkat cells were incubated with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01), resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-2-UNLB (SB Cat. No. 17100-01). Proteins were visualized using Goat Anti-Rat IgM-HRP (SB Cat. No. 3020-05) secondary antibody and chemiluminescent detection.
Total cell lysates from Jurkat cells were incubated with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01), resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-2-UNLB (SB Cat. No. 17100-01). Proteins were visualized using Goat Anti-Rat IgM-HRP (SB Cat. No. 3020-05) secondary antibody and chemiluminescent detection.
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