Hamster Anti-Mouse TCRβ-Alexa Fluor® 647

Cat. No.
1785-31  
Size
0.1 mg   		Price (USD)
$190.00 
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Clone H57-597
Isotype Hamster (Armenian) IgG2
Isotype Control Hamster IgG-Alexa Fluor® 647
Immunogen TCR affinity purified from mouse T-cell hybridoma DO-11.10
Specificity Mouse TCRβ
Description The αβ TCR is expressed on T lymphocytes of all mouse strains tested. The H57-597 monoclonal antibody does not react with γδ TCR-bearing cells. Plate-bound or soluble H57-597 activates αβ TCR-bearing T cells. In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis. In vivo administration of H57-597 has been shown to deplete αβ TCR-bearing cells to near completion and prevent graft rejection.
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,8-12
Immunohistochemistry –Reported in literature 7
Immunocytochemistry – Reported in literature 6
Immunoprecipitation – Reported in literature 1
Stimulation – Reported in literature 1,2
Depletion – Reported in literature 3,4
Separation – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795290
Gene ID
21577 (Mouse)
Gene Symbol
Tcrb (Mouse)
Gene Aliases TCRbeta; Tib
  • Hamster Anti-Mouse TCRb-AF647
    C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse TCRβ-AF647 (SB Cat. No. 1785-31) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).

C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse TCRβ-AF647 (SB Cat. No. 1785-31) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
 
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References
1. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine αβ T cell receptors. J Immunol. 1989;142:2736-42. (Immunogen, FC, IP, Stim)
2. Zerrahn K, Held W, Raulet DH. The MHC reactivity of the T cell repertoire prior to positive and negative selection. Cell. 1997;88:627-36. (Stim)
3. Davenport C, Kumar V, Bennett M. Rapid rejection of H2k and H2k/b bone marrow cell grafts by CD8+ T cells and NK cells in irradiated mice. J Immunol. 1995;155:3742-9. (Depletion)
4. Atsuta N, Nishimura H, Nakamura N, Emoto M, Iwatsuki T, Yoshokai Y. Diversity of Vγ gene segments rearranged to the Jγ4 gene in mice. J Immunol. 1995;154:676-84. (Depletion)
5. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (Sep)
6. Dong L, Watanabe K, Itoh M, Huan C, Tong X, Nakamura T, et al. CD4+ T-cell dysfunctions through the impaired lipid rafts ameliorate concanavalin A-induced hepatitis in sphingomyelin synthase 1-knockout mice. Int Immunol. 2012;24:327-37. (ICC)
7. Chatenoud L, Thervet E, Primo J, Bach J. Anti-CD3 antibody induces long-term remission of overt autoimmunity in nonobese diabetic mice. Proc Natl Acad Sci USA. 1994;91:123-7. (IHC)
8. Buslepp J, Kerry SE, Loftus D, Frelinger JA, Appella E, Collins EJ. High affinity xenoreactive TCR:MHC interaction recruits CD8 in absence of binding to MHC. J Immunol. 2003;170:373-83. (FC)
9. Galkina E, Tanousis K, Preece G, Tolaini M, Kioussis D, Florey O, et al. L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes. J Exp Med. 198;9:1323-35. (FC)
10. Rodríguez-Borlado L, Barber DF, Hernández C, Rodríguez-Marcos MA, Sánchez S, Hirsch E, et al. Phosphatidylinositol 3-kinase regulates the CD4/CD8 T cell differentiation ratio. J Immunol. 2003;170:4475-82. (FC)
11. Woolf E, Xiao C, Fainaru O, Lotem J, Rosen D, Negreanu V, et al. Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis. Proc Natl Acad Sci USA. 2003;100:7731-6. (FC)
12. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC, Porcupine Reactivity)
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