Ensuring timely antibody supply during the COVID-19 pandemic - contact us for solutions

Hamster Anti-Mouse TCRβ-BIOT (H57-597)

Cat. No.:
Biotin Anti-Mouse TCRβ antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and separation assays.
Size Price (USD) Quantity
0.5 mg $212.00
More Information
Clone H57-597
Isotype Hamster (Armenian) IgG2
Isotype Control Hamster IgG-BIOT
Specificity Mouse TCRβ
Description The αβ TCR is expressed on T lymphocytes of all mouse strains tested. The H57-597 monoclonal antibody does not react with γδ TCR-bearing cells. Plate-bound or soluble H57-597 activates αβ TCR-bearing T cells. In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis. In vivo administration of H57-597 has been shown to deplete αβ TCR-bearing cells to near completion and prevent graft rejection.
Immunogen TCR affinity purified from mouse T-cell hybridoma DO-11.10
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 1,8-12
Immunohistochemistry –Reported in literature 7
Immunocytochemistry – Reported in literature 6
Immunoprecipitation – Reported in literature 1
Stimulation – Reported in literature 1,2
Depletion – Reported in literature 3,4
Separation – Reported in literature 5

RRID Number AB_2795281
Gene ID 21577 (Mouse)
Gene ID Symbol Tcrb (Mouse)
Gene ID Aliases TCRbeta; Tib

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Need help with this product?
or call 800.722.2255
  1. 1. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine αβ T cell receptors. J Immunol. 1989;142:2736-42. (Immunogen, FC, IP, Stim)
  2. 2. Zerrahn K, Held W, Raulet DH. The MHC reactivity of the T cell repertoire prior to positive and negative selection. Cell. 1997;88:627-36. (Stim)
  3. 3. Davenport C, Kumar V, Bennett M. Rapid rejection of H2k and H2k/b bone marrow cell grafts by CD8+ T cells and NK cells in irradiated mice. J Immunol. 1995;155:3742-9. (Depletion)
  4. 4. Atsuta N, Nishimura H, Nakamura N, Emoto M, Iwatsuki T, Yoshokai Y. Diversity of Vγ gene segments rearranged to the Jγ4 gene in mice. J Immunol. 1995;154:676-84. (Depletion)
  5. 5. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (Sep)
  6. 6. Dong L, Watanabe K, Itoh M, Huan C, Tong X, Nakamura T, et al. CD4+ T-cell dysfunctions through the impaired lipid rafts ameliorate concanavalin A-induced hepatitis in sphingomyelin synthase 1-knockout mice. Int Immunol. 2012;24:327-37. (ICC)
  7. 7. Chatenoud L, Thervet E, Primo J, Bach J. Anti-CD3 antibody induces long-term remission of overt autoimmunity in nonobese diabetic mice. Proc Natl Acad Sci USA. 1994;91:123-7. (IHC)
  8. 8. Buslepp J, Kerry SE, Loftus D, Frelinger JA, Appella E, Collins EJ. High affinity xenoreactive TCR:MHC interaction recruits CD8 in absence of binding to MHC. J Immunol. 2003;170:373-83. (FC)
  9. 9. Galkina E, Tanousis K, Preece G, Tolaini M, Kioussis D, Florey O, et al. L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes. J Exp Med. 198;9:1323-35. (FC)
  10. 10. Rodríguez-Borlado L, Barber DF, Hernández C, Rodríguez-Marcos MA, Sánchez S, Hirsch E, et al. Phosphatidylinositol 3-kinase regulates the CD4/CD8 T cell differentiation ratio. J Immunol. 2003;170:4475-82. (FC)
  11. 11. Woolf E, Xiao C, Fainaru O, Lotem J, Rosen D, Negreanu V, et al. Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis. Proc Natl Acad Sci USA. 2003;100:7731-6. (FC)
  12. 12. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC, Porcupine Reactivity)
See All References