Hamster Anti-Mouse Ly-49C/F/H/I-UNLB
Cat. No.
1801-01
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Size
0.5 mg
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Price (USD)
$198.00
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Clone |
14B11
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Isotype |
Hamster (Syrian) IgG1
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Isotype Control |
Hamster IgG-UNLB
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Immunogen |
IL-2 activated natural killed (A-LAK) cells from C57BL/6 mice
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Specificity |
Mouse Ly-49C/F/H/I
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Alternalte Name(s) |
Ly-49
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Description |
The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H, and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells while allowing them to attack infected or transformed cells in which class I molecules have been downregulated. These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains ~90% of NK1.1+CD3- splenocytes and ~6% of CD8+ spleen cells from C57BL/6 mice. The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).
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Format/Conjugate |
UNLB (Unconjugated)
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Buffer Formulation |
Borate buffered saline, pH 8.2
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1,2 Cytoxicity – Reported in literature 1
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795327
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Gene ID |
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Gene Symbol |
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Gene Aliases |
5E6; Ly49c; NK-2.1; Nk-2; Nk2; Nk2.1; Ly49f; ly-49f; Cmv-1; Cmv1; Ly49h; Ly49u<129>; LY49I1; Ly49I
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Uniprot ID |
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Uniprot Name |
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-
C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse Ly-49C/F/H/I-PE (SB Cat. No. 1801-09) and Mouse Anti-Mouse NK1.1-FITC (SB Cat. No. 1805-02).
C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse Ly-49C/F/H/I-PE (SB Cat. No. 1801-09) and Mouse Anti-Mouse NK1.1-FITC (SB Cat. No. 1805-02).
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References
1. | Corral L, Takizawa H, Hanke T, Jamieson AM, Raulet DH. A new monoclonal antibody reactive with several Ly49 NK cell receptors mediates redirected lysis of target cells. Hybridoma. 1999;18:359-66. (Immunogen, Cyto, FC) | 2. | Chung JW, Kim MS, Piao Z, Jeong M, Yoon SR, Shin N, et al. Osteopontin promotes the development of natural killer cells from hematopoietic stem cells. Stem Cells. 2008;26:2114-23. (FC) |
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