Hamster Anti-Mouse CD81-BIOT

Cat. No.
1825-08  
Size
0.5 mg  
Price (USD)
$245.00 
Clone 2F7
Isotype Hamster (Armenian) IgG3
Isotype Control Hamster IgG-BIOT
Immunogen Mouse epithelial cell line PAM212
Specificity Mouse CD81
Alternalte Name(s) TAPA-1
Description CD81, also known as TAPA-1, is an integral membrane protein expressed on a variety of cell types and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of γδ+ T cells to more mature T cells with αβ T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor which is composed of a TCR β chain and glycoprotein pTα. The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3
Immunohistochemistry –Reported in literature 1
Immunocytochemistry – Reported in literature 5
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1-4
Costimulation – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795400
Gene ID
12520 (Mouse)
Gene Symbol
Cd81 (Mouse)
Gene Aliases Tapa-1; Tapa1; Tspan28
Uniprot ID
P35762 (Mouse)
Uniprot Name
  • Chinese hamster ovary cell line CHO-K1 and BALB/cAnN mouse B lymphocyte cell line A20 were stained with Hamster Anti-Mouse CD81-BIOT (SB Cat. No. 1825-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Chinese hamster ovary cell line CHO-K1 and BALB/cAnN mouse B lymphocyte cell line A20 were stained with Hamster Anti-Mouse CD81-BIOT (SB Cat. No. 1825-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Boismenu R, Rhein M, Fischer WH, Havran WL. A role for CD81 in early T cell development. Science. 1996;271:198-200. (Immunogen, FC, IP, IHC, Block)
2. Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97. (Block, Costim)
3. Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999;146:893-904. (FC, Block)
4. Chang Y, Finnemann SC. Tetraspanin CD81 is required for the αvβ5-integrin-dependent particle-binding step of RPE phagocytosis. J Cell Sci. 2007;120:3053-63. (Block)
5. Kelić S, Levy S, Suarez C, Weinstein DE. CD81 regulates neuron-induced astrocyte cell-cycle exit. Mol Cell Neurosci. 2001;17:551-60. (ICC)