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Mouse Anti-Mouse I-Ek-PE (17-3-3)
Cat. No.:
1898-09
PE Anti-Mouse I-Ek antibody for use in flow cytometry assays.
$180.00
| Clone | 17-3-3 |
|---|---|
| Isotype | Mouse (C3H.SW) IgG2aκ |
| Isotype Control | Mouse IgG2a-PE (HOPC-1) |
| Specificity | Mouse I-Ek |
| Alternative Names | MHC Class II |
| Description | The monoclonal antibody 17-3-3 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype. Reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g, H-2q, and H-2s haplotypes do not express I-E antigen. |
| Immunogen | C3H mouse skin graft and splenocytes |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications | Flow Cytometry – Quality tested 3 Immunohistochemistry-Frozen Sections – Reported in literature 2 Complement Mediated Cell Depletion – Reported in literature 1 |
| RRID Number | AB_2795461 |
| Gene ID | 14969 (Mouse) |
| Gene ID Symbol | H2-Eb1 (Mouse) |
| Gene ID Aliases | Eb; Ia4; H2Eb; Ia-4; H-2Eb |
| UniProt ID | P18469 (Mouse |
| UniProt Name | HB2J_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC)
- 2. Colson YL, Lange J, Fowler K, Ildstad ST. Mechanism for cotolerance in nonlethally conditioned mixed chimeras: negative selection of the Vbeta T-cell receptor repertoire by both host and donor bone marrow-derived cells. Blood. 1996;88:4601-10. (IHC-FS)
- 3. Anderson GD, David CS. In vivo expression and function of hybrid Ia dimers (EαAβ) in recombinant and transgenic mice. J Exp Med. 1989;170:1003-8. (FC)
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