Mouse Anti-Mouse H-2Dd-BIOT

Cat. No.
1912-08  
Size
0.5 mg   		Price (USD)
$225.00 
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Clone 34-5-8S
Isotype Mouse (C3H/HeJ) IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen BDF1 mouse splenocytes
Specificity Mouse H-2Dd
Alternalte Name(s) MHC Class I
Description The monoclonal antibody 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of α1 and α2 chains when complexed with β2-microglobulin. The antibody does not react with H-2Dd α chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 8
Immunocytochemistry – Reported in literature 8
Immunoprecipitation – Reported in literature 4,5,8
Complement Mediated Cell Depletion – Reported in literature 1
Adhesion – Reported in literature 2,3
Blocking – Reported in literature 2,3
Purification – Reported in literature 2,6
ELISA – Reported in literature 6,7
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795502
Gene ID
14964 (Mouse)
Gene Symbol
H2-D1 (Mouse)
Gene Aliases H-2D; H2-D; H2-K1
Uniprot ID
P01900 (Mouse)
Uniprot Name
HA12_MOUSE 
  • Mouse Anti-Mouse H-2Dd-BIOT
    DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse H-2Dd-BIOT (SB Cat. No. 1912-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse H-2Dd-BIOT (SB Cat. No. 1912-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Ozato K, Mayer NM, Sachs DH. Monoclonal antibodies to mouse major histocompatibility complex antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation. 1982;34:113-9. (Immunogen, CMDC)
2. Kane KP. Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules. J Exp Med. 1994;179:1011-5. (Block, Purification, Adhesion)
3. Brennan J, Mahon G, Mager DL, Jefferies WA, Takei F. Recognition of class I major histocompatibility complex molecules by Ly-49: specificities and domain interactions. J Exp Med. 1996;183:1553-9. (Block, Adhesion)
4. Beck JC, Hansen TH, Cullen SE, Lee DR. Slower processing, weaker β2-M association, and lower surface expression of H-2Ld are influenced by its amino terminus. J Immunol. 1986;137:916-23. (IP)
5. Lie W, Myers NB, Connolly JM, Gorka J, Lee DR, Hansen TH. The specific binding of peptide ligand to Ld class I major histocompatibility complex molecules determines their antigenic structure. J Exp Med. 1991;173:449-59. (IP)
6. White J, Crawford F, Fremont D, Marrack P, Kappler J. Soluble class I MHC with β2-microglobulin covalently linked peptides: specific binding to a T cell hybridoma. J Immunol. 1999;162:2671-6. (Purification, ELISA)
7. Mage MG, Lee Li, Ribaudo RK, Corr M, Kozlowski S, McHugh L, et al. A recombinant, soluble, single-chain class I major histocompatibility complex molecule with biological activity. Proc Natl Acad Sci USA. 1992;89:10658-62. (ELISA)
8. Paquet M, Cohen-Doyle M, Shore GC, Williams DB. Bap29/31 influences the intracellular traffic of MHC class I molecules. J Immunol. 2004;172:7548-55. (IP, ICC, FC)