Mouse Anti-Mouse H-2Db-BIOT

Cat. No.
1913-08  
Size
0.5 mg  
Price (USD)
$235.00 
Clone 27-11-13S
Isotype Mouse (C3H) IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen BDF1 mouse splenocytes
Specificity Mouse H-2Db
Alternalte Name(s) MHC Class I
Description The monoclonal antibody 27-11-13S recognizes with the α3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the α3 domain of H-2Dd, H-2Dq, H-2Ld and H-2Lq but not H-2Kb. Reactivity with haplotypes k, f, p, r, and s has not been observed.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 4
Complement Mediated Cell Depletion – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795506
Gene ID
14964 (Mouse)
Gene Symbol
H2-D1 (Mouse)
Gene Aliases H-2D; H2-D; H2-K1
Uniprot ID
P01899 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Db-BIOT (SB Cat. No. 1913-08) followed by Streptavidin-FITC (SB Cat No. 7100-02).

BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Db-BIOT (SB Cat. No. 1913-08) followed by Streptavidin-FITC (SB Cat No. 7100-02).
 
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References
1. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981;126:317-21. (Immunogen, CMDC)
2. Palmowski MJ, Parker M, Choudhuri K, Chiu C, Callan MF, van der Merwe PA. A single-chain H-2Db molecule presenting an influenza virus nucleoprotein epitope shows enhanced ability at stimulating CD8+ T cell responses in vivo. J Immuno. 2009;182:4565-71. (FC)
3. Choudhuri K, Parker M, Milicic A, Cole DK, Shaw MK, Sewell AK, et al. Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem. 2009;284:26096-105. (ICC)
4. Shemesh J, Ehrlich R. Aberrant biosynthesis and transport of class I major histocompatibility complex molecules in cells transformed with highly oncogenic human adenoviruses. J Biol Chem. 1993;268:15704-11. (IP)