Mouse Anti-Mouse H-2Dk-BIOT

Cat. No.
1914-08  
Size
0.5 mg   		Price (USD)
$275.00 
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Clone 15-5-5
Isotype Mouse (C3H.SW) IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen C3H mouse splenocytes
Specificity Mouse H-2Dk
Alternalte Name(s) MHC Class I
Description The monoclonal antibody 15-5-5 reacts with the H-2Dk class I alloantigen. It cross-reacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 6
Immunoprecipitation – Reported in literature 3
Complement Mediated Cell Depletion – Reported in literature 1,2
ELISA – Reported in literature 4
Blocking – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795512
Gene ID
14964 (Mouse)
Gene Symbol
H2-D1 (Mouse)
Gene Aliases H-2D; H2-D; H2-K1
Uniprot ID
P14426 (Mouse)
Uniprot Name
HA13_MOUSE 
  • Mouse Anti-Mouse H-2Dk-BIOT
    AKR mouse splenocytes were stained with Mouse Anti-Mouse H-2Dk-BIOT (SB Cat. No. 1914-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

AKR mouse splenocytes were stained with Mouse Anti-Mouse H-2Dk-BIOT (SB Cat. No. 1914-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC)
2. Rogers MJ, Gooding LR, Margulies DH, Evans GA. Analysis of a defect in the H-2 genes of SV40 transformed C3H fibroblasts that do not express H-2Kk. J Immunol. 1983;130:2418-22. (CMDC)
3. Houlden BA, Widacki SM, Bluestone JA. Signal transduction through class I MHC by a monoclonal antibody that detects multiple murine and human class I molecules. J Immunol. 1991;146:425-30. (IP)
4. Bedford P, Garner K, Knight SC. MHC class II molecules transferred between allogeneic dendritic cells stimulate primary mixed leukocyte reactions. Int Immunol. 1999;11:1739-44. (ELISA)
5. Desrosiers M, Kielczewska A, Loredo-Osti J, Adam SG, Makrigiannis AP, Lemieux S, et al. Epistasis between mouse Klra and major histocompatibility complex class I loci is associated with a new mechanism of natural killer cell-mediated innate resistance to cytomegalovirus infection. Nat Genet. 2005;37:593-9. (Block)
6. Matsuoka S, Asano Y, Sano K, Kishimoto H, Yamashita I, Yorifuji H, et al. A novel type of cell death of lymphocytes induced by a monoclonal antibody without participation of complement. J Exp Med. 1995;181:2007-15. (FC)