Goat Anti-Human Ig Fc-AP

Cat. No.
2047-04  
Size
1.0 mL  
Price (USD)
$140.00 
Specificity Reacts with the Fc region of human IgG, IgM, and IgA
Source Pooled antisera from goats hyperimmunized with human IgG, IgM, and IgA
Cross Adsorption Human IgG Fab; may react with immunoglobulins from other species and the Fc region of other human immunoglobulins
Purification Affinity chromatography on pooled human immunoglobulins covalently linked to agarose
Isotype Goat IgG
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795682
  • ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human Ig Fc-AP (SB Cat. No. 2047-04).

ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human Ig Fc-AP (SB Cat. No. 2047-04).
 
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References
1. Terato K, Do CT, Cutler D, Waritani T, Shionoya H. Preventing intense false positive and negative reactions attributed to the principle of ELISA to re-investigate antibody studies in autoimmune diseases. J Immunol Methods. 2014;407:15-25. (ELISA)
2. Zhao Q, Ahmed M, Guo H, Cheung IY, Cheung NV. Alteration of electrostatic surface potential enhances affinity and tumor killing properties of anti-ganglioside GD2 monoclonal antibody hu3F8. J Biol Chem. 2015;290:13017-27. (ELISA)