Goat Anti-Rat Ig, Mouse ads-HRP

Cat. No.
3010-05  
Size
1.0 mL  
Price (USD)
$165.00 
Specificity Reacts with the heavy and light chains of rat IgG and IgM
Source Pooled antisera from goats hyperimmunized with rat IgG and IgM
Cross Adsorption Mouse immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled rat Ig covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Flow Cytometry 3-12
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1,13-15
Immunohistochemistry-Paraffin Sections 16,17
Immunocytochemistry 1,4,9
Western Blot 1,2,18-21
Depletion 22
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795801
  • ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05).
  • Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05) and chemiluminescent detection.

    Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)
  • ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-UNLB (SB Cat. No. 1140-01) followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05).

ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05).
 

Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05) and chemiluminescent detection.

Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)
 

ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-UNLB (SB Cat. No. 1140-01) followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05).
 
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References
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