Mouse Anti-Rabbit Light Chain-HRP

Cat. No.
4060-05  
Size
1.0 mL  
Price (USD)
$275.00 
Clone SB62a
Isotype Mouse (BALB/c) IgG2bλ
Isotype Control Mouse IgG2b-HRP
Immunogen Rabbit light chains
Specificity Rabbit light chains
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
Western Blot – Reported in literature 2-4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795985
  • Reduced and non-reduced rabbit IgG was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05). Light chains were visualized using chemiluminescent detection.
  • ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05).
  • Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05) followed by chemiluminescent detection.

Reduced and non-reduced rabbit IgG was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05). Light chains were visualized using chemiluminescent detection.
 

ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05).
 

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05) followed by chemiluminescent detection.
 
Related Products (20)
References
1. Zhang Y, Phung Y, Gao W, Kawa S, Hassan R, Pastan I, et al. New high affinity monoclonal antibodies recognize non-overlapping epitopes on mesothelin for monitoring and treating mesothelioma. Sci Rep. 2015;5:09928. (ELISA)
2. Wu X, Liu L, Zhang Z, Zhang B, Sun H, Chan GL, et al. Selective protection of normal cells during chemotherapy by RY4 peptides. Mol Cancer Res. 2014;12:1365-76. (WB)
3. Bi H, Li S, Qu X, Wang M, Bai X, Xu Z, et al. DEC1 regulates breast cancer cell proliferation by stabilizing cyclin E protein and delays the progression of cell cycle S phase. Cell Death Dis. 2015;6:e1891. (WB)
4. Chuprin A, Avin A, Goldfarb Y, Herzig Y, Levi B, Jacob A, et al. The deacetylase Sirt1 is an essential regulator of Aire-mediated induction of central immunological tolerance. Nat Immunol. 2015;16:737-45. (WB)