Mouse Anti-Porcine CD4-SPRD

Cat. No.
4515-13  
Size
0.1 mg  
Price (USD)
$225.00 
Clone 74-12-4
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-SPRD
Immunogen Fresh dd miniature swine thymocytes
Specificity Porcine CD4
Description Porcine CD4 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. CD4 is an accessory molecule in the recognition of foreign antigens in association with MHC Class II molecules by T lymphocytes.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,6,7,15,17-31
Immunohistochemistry-Frozen Sections – Reported in literature 2-8
Immunohistochemistry-Paraffin Sections – Reported in literature 9-10
Immunocytochemistry – Reported in literature 32
Immunoprecipitation – Reported in literature 1
Separation – Reported in literature 11-14
Blocking – Reported in literature 15,16
Complement Mediated Cell Depletion – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796028
Gene ID
404704 (Porcine)
Gene Symbol
CD4 (Porcine)
  • Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD4-SPRD (SB Cat. No. 4515-13) and Mouse Anti-Porcine CD8α-PE (SB Cat. No. 4520-09).

Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD4-SPRD (SB Cat. No. 4515-13) and Mouse Anti-Porcine CD8α-PE (SB Cat. No. 4520-09).
 
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References
1. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984;133:368-375. (Immunogen, FC, IP, CMDC)
2. Minguez I, Rueda A, Domínguez J, Sánchez-Vizcaíno JM. Double labeling immunohistological study of African swine fever virus-infected spleen and lymph nodes. Vet Pathol. 1988;25:193-8. (IHC-FS)
3. Yamada K, Shimizu A, Ierino FL, Utsugi R, Barth RN, Esnaola N, et al. Thymic transplantation in miniature swine. I. Development and function of the "thymokidney". Transplantation. 1999;68:1684-92. (IHC-FS)
4. Shimizu A, Yamada K, Meehan SM, Sachs DH, Colvin RB. Acceptance reaction: intragraft events associated with tolerance to renal allografts in miniature swine. J Am Soc Nephrol. 2000;11:2371-80. (IHC-FS)
5. Shimizu A, Yamada K, Sachs DH, Colvin RB. Mechanisms of chronic renal allograft rejection. II. Progressive allograft glomerulopathy in miniature swine. Lab Invest. 2002;82:673-85. (IHC-FS)
6. Jamin A, Gorin S, Le Potier M, Kuntz-Simon G. Characterization of conventional and plasmacytoid dendritic cells in swine secondary lymphoid organs and blood. Vet Immunol Immunopathol. 2006;114:224-37. (FC, IHC-FS)
7. Jamin A, Gorin S, Cariolet R, Le Potier M, Kuntz-Simon G. Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs. Vet Res. 2008;39:7. (FC, IHC-FS)
8. Debeer S, Le Luduec J, Kaiserlian D, Laurent P, Nicolas J, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dematol. 2013;23:456-66. (IHC-FS)
9. Tingstedt J, Nielsen J. Cellular immune responses in the lungs of pigs infected in utero with PRRSV: an immunohistochemical study. Viral Immunol. 2004;17:558-64. (IHC-PS)
10. Jung K, Alekseev KP, Zhang X, Cheon D, Vlasova AN, Saif LJ. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol. 2007;81:13681-93. (IHC-PS)
11. Toka FN, Nfon CK, Dawson H, Golde WT. Accessory-cell-mediated activation of porcine NK cells by toll-like receptor 7 (TLR7) and TLR8 agonists. Clin Vaccine Immunol. 2009;16:866-78. (Sep)
12. Toka FN, Nfon CK, Dawson H, Estes DM, Golde WT. Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells. J Interferon Cytokine Res. 2009;29:179-92. (Sep)
13. Toka FN, Nfon C, Dawson H, Golde WT. Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus. Clin Vaccine Immunol. 2009;16:1738-49. (Sep)
14. Kreuzer S, Rieger J, Strucken EM, Thaben N, Hünigen H, Nöckler K, et al. Characterization of CD4+ subpopulations and CD25+ cells in ileal lymphatic tissue of weaned piglets infected with Salmonella Typhimurium with or without Enterococus faecium feeding. Vet Immunol Immunopathol. 2014;158:143-55. (FC, Sep)
15. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985;134:37-44. (Block, FC)
16. Saíz JC, Rodríguez A, González M, Alonso F, Sobrino F. Heterotypic lymphoproliferative response in pigs vaccinated with foot-and-mouth disease virus. Involvement of isolated capsid proteins. J Gen Virol. 1992;73:2601-7. (Block)
17. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
18. Layton DS, Bean AG, Dodge NM, Strom AD, Sandrin MS, Ierino FL. Differential cytokine expression and regulation of human anti-pig xenogeneic responses by modified porcine dendritic cells. Xenotransplantation. 2008;15:257-67. (FC)
19. Martelli P, Gozio S, Ferrari L, Rosina S, De Angelis E, Quintavalla C, et al. Efficacy of a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: Clinical protection and cell-mediated immunity. Vaccine. 2009;27:3788-99. (FC)
20. Ferrari L, Borghetti P, Gozio S, De Angelis E, Ballotta L, Smeets J, et al. Evaluation of the immune response induced by intradermal vaccination by using a needle-less system in comparison with the intramuscular route in conventional pigs. Res Vet Sci. 2011;90:64-71. (FC)
21. Oh Y, Seo HW, Han K, Park C, Chae C. Protective effect of the maternally derived porcine circovirus type 2 (PCV2)-specific cellular immune response in piglets by dam vaccination against PCV2 challenge. J Gen Virol. 2012;93:1556-62. (FC)
22. Ravlo K, Koefoed-Nielsen P, Secher N, Søndergaard P, Keller AK, Petersen MS, et al. Effect of remote ischemic conditioning on dendritic cell number in blood after renal transplantation - flow cytometry in a porcine model. Transpl Immunol. 2012;26:146-50. (FC)
23. Hester SN, Comstock SS, Thorum SC, Monaco MH, Pence BD, Woods JA, et al. Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets. Clin Vaccine Immunol. 2012;19:1499-508. (FC)
24. Villena J, Suzuki R, Fujie H, Chiba E, Takahashi T, Tomosada Y, et al. Immunobiotic Lactobacillus jensenii modulates the Toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen-presenting cells. Clin Vaccine Immunol. 2012;19:1038-53. (FC)
25. Liu Y, Che TM, Song M, Lee JJ, Almeida JA, Bravo D, et al. Dietary plant extracts improve immune responses and growth efficiency of pigs experimentally infected with porcine reproductive and respiratory syndrome virus. J Anim Sci. 2013;91:5668-79. (FC)
26. Liu KY, Comstock SS, Shunk JM, Monaco MH, Donovan SM. Natural killer cell populations and cytotoxic activity in pigs fed mother's milk, formula, or formula supplemented with bovine lactoferrin. Pediatr Res. 2013;74:402-7. (FC)
27. Thorum SC, Hester SN, Comstock SS, Monaco MH, Pence BD, Woods JA, et al. Dietary (1,3/1,6)-β-D-glucan decreases transforming growth factor β expression in the lung of the neonatal piglet. Nutr Res. 2013;33:322-31 (FC)
28. Liu P, Pieper R, Tedin L, Martin L, Meyer W, Rieger J, et al. Effect of dietary zinc oxide on jejunal morphological and immunological characteristics in weaned piglets. J Anim S. 2014;92:5009-18. (FC)
29. Ferrari L, Borghetti P, De Angelis E, Martelli P. Memory T cell proliferative responses and IFN-γ productivity sustain long-lasting efficacy of a Cap-based PCV2 vaccine upon PCV2 natural infection and associated disease. Vet Res. 2014;45:44. (FC)
30. Leclercq C, Prunier A, Thomas F, Merlot E. Neonatal surgical castration of male pigs reduces thymic growth but has moderate consequences on thymocytes. J Anim Sci. 2014;92:2415-21. (FC)
31. Whelan RA, Rausch S, Ebner F, Günzel D, Richter JF, Hering NA, et al. A transgenic probiotic secreting a parasite immunomodulator for site-directed treatment of gut inflammation. Mol Ther. 2014;22:1730-40. (FC)
32. Matsubara T, Nishii N, Takashima S, Takasu M, Imaeda N, Aiki-Oshima K, et al. Identification and characterization of two CD4 alleles in Microminipigs. BMC Vet Res. 2016;12:222. (ICC)
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