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Mouse Anti-Porcine CD21-AF488 (BB6-11C9.6)

Cat. No.:
Alexa Fluor® 488 Anti-Porcine CD21 for use in flow cytometry and immunohistochemistry assays.
Size Price (USD) Quantity
0.1 mg $270.00
More Information
Clone BB6-11C9.6
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-AF488 (15H6)
Specificity Porcine CD21
Description CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. Low density CD21 has also been reported on granulocytes and monocytes. The monoclonal antibody BB6-11C9.6 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues - 92-96% in peripheral blood, 74-98% in mesenteric lymph nodes, 97-100% in ileal Peyer’s patches (ILPP), 93-97% in spleen, and 0% in thymus. The antibody does not stain B cells in cord blood or spleen from pig fetuses (105 days gestation).
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 4,8-21
Immunohistochemistry-Frozen Sections – Reported in literature 1-3
Immunoprecipitation – Reported in literature 4
Separation – Reported in literature 5-8

RRID Number AB_2796046

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  2. 2. Jamin A, Gorin S, Le Potier M, Kuntz-Simon G. Characterization of conventional and plasmacytoid dendritic cells in swine secondary lymphoid organs and blood. Vet Immunol Immunopathol. 2006;114:224-37. (IHC-FS)
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  7. 7. Toka FN, Nfon CK, Dawson H, Golde WT. Accessory-cell-mediated activation of porcine NK cells by toll-like receptor 7 (TLR7) and TLR8 agonists. Clin Vaccine Immunol. 2009;16:866-78. (Sep)
  8. 8. Liu KY, Comstock SS, Shunk JM, Monaco MH, Donovan SM. Natural killer cell populations and cytotoxic activity in pigs fed mother's milk, formula, or formula supplemented with bovine lactoferrin. Pediatr Res. 2013;74:402-7. (FC, Sep)
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  15. 15. Shi K, Li H, Guo X, Ge X, Jia H, Zheng S, et al. Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet Microbiol. 2008;129:367-77. (FC)
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  19. 19. Liu Y, Che TM, Song M, Lee JJ, Almeida JA, Bravo D, et al. Dietary plant extracts improve immune responses and growth efficiency of pigs experimentally infected with porcine reproductive and respiratory syndrome virus. J Anim Sci. 2013;91:5668-79. (FC)
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