Rabbit F(ab')2 Anti-Human IgG(H+L), Mouse ads-UNLB

Cat. No.
6005-01  
Size
0.5 mg  
Price (USD)
$95.00 
Specificity Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Source Pepsin digest of Rabbit Anti-Human IgG(H+L), Mouse ads (SB Cat. No. 6145)
Cross Adsorption Mouse immunoglobulins and pooled sera; may react with immunoglobulins from other species
Isotype Rabbit F(ab')2 IgG
Isotype Control Rabbit F(ab')2 IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796087
  • ELISA plate was coated with purified human IgG, IgM, and IgA and mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit F(ab')2 Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6005-01) followed by Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).

ELISA plate was coated with purified human IgG, IgM, and IgA and mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit F(ab')2 Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6005-01) followed by Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
 
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References
1. Baselmans PJ, Pöllabauer E, van Reijsen FC, Heystek HC, Hren A, Stumptner P, et al. IgE production after antigen-specific and cognate activation of HLA-DPw4-restricted T-cell clones, by 78% of randomly selected B-cell donors. Hum Immunol. 2000;61:789-98. (ELISA)
2. Piesche M, Ho VT, Kim H, Nakazaki Y, Nehil M, Yaghi NK, et al. Angiogenic cytokines are antibody targets during graft-versus-leukemia reactions. Clin Cancer Res. 2015;21:1010-8. (ELISA)